sy680 is a novel allele of pkd-2.

Description

Summary a new allele of was isolated in a behavioral genetic screen for male mating defects, and found to result in a substitution of Arginine for Glycine in the equivalent of human PKD2 alanine 615.

ArticleThe C. elegans ortholog of polcystin-2 is encoded by (Barr et al., 2001). From an EMS screen of a strain for male mating defective mutants and a secondary behavioral screen for defects in discrete steps of male mating behavior, namely response to contact to hermaphrodites and vulval location (described in Schindelman et al., 2006), we identified a new allele of based on mapping and complementation. fails to complement () for defects in response to contact with hermaphrodite and vulval location. Here we report the sequence of this allele. PCR amplification and sequencing of exons indicated that there was a c–>t transition in the transcribed DNA strand (g–>a in the sense strand; Figure 1A). This change leads to an altered codon, a Glycine to Arginine substitution the PKD-2 protein. This position corresponds to A615 of the human protein (Figure 1B).

Figure 1.

A. The position of lesion in the pkd-2 DNA sequence. B. Location of lesion in an alignment of C. elegansand human proteins. Position of substitution shown in red.

Reagents

Strains:PS7518 (e2001d) III; () IV; () V

PS3400 ()