Zhen Liang1, Yuzhu Tang2, Hao Li3, Youjun Xie4, Lingling Zhan4. 1. Department of Clinical Laboratory, Red Cross Hospital of Yulin City, Yulin, China. 2. Department of Clinical Laboratory, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, China. 3. Department of Blood Transfusion, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China. 4. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Abstract
BACKGROUND: To investigate the association between phosphatase and tension homologue deleted on chromosome ten (PTEN) gene polymorphisms and non-small-cell lung cancer (NSCLC) and further identify whether these polymorphisms influence serum PTEN levels. METHODS: A total of 152 NSCLC patients and 124 healthy controls were included in the study. PTEN gene rs11202586 (T > C) and rs1903858 (A > G) polymorphisms were detected using the multiple single-base extension technique (SNaPshot). The serum PTEN levels were determined using an enzyme-linked immunosorbent assay (ELISA) kit. RESULTS: The rs1903858 AG, GG genotypes, and G allele were associated with a higher risk of NSCLC (odds ratio (OR) =2.079, 95% confidence interval (CI) = 1.087-3.974, P = .027; OR = 1.897, 95%CI = 1.053-3.419, P = .033; OR = 1.505, 95%CI = 1.065-2.126, P = .020). Stratified analysis reveal that the rs1903858 GG genotype and G allele were associated with an increased risk of squamous cell carcinoma (SCC) (OR = 3.226, 95%CI = 1.075-9.678, P = .037; OR = 1.873, 95%CI = 1.092-3.212, P = .023). Among smokers, the rs1903858 G allele carriers have an increased risk of NSCLC (OR = 1.916, 95%CI = 1.023-3.589, P = .042), but a decreased risk of NSCLC was found with the AT haplotype. With respect to the serum PTEN levels, no significant difference was noted between NSCLC patients and healthy controls in this study. CONCLUSIONS: The study indicated that the rs1903858 gene polymorphism is associated with increased risk of NSCLC, particularly in SCC and smoker, and the haplotype AT was a protective factor for NSCLC. The serum PTEN levels were not associated with NSCLC.
BACKGROUND: To investigate the association between phosphatase and tension homologue deleted on chromosome ten (PTEN) gene polymorphisms and non-small-cell lung cancer (NSCLC) and further identify whether these polymorphisms influence serum PTEN levels. METHODS: A total of 152 NSCLCpatients and 124 healthy controls were included in the study. PTEN gene rs11202586 (T > C) and rs1903858 (A > G) polymorphisms were detected using the multiple single-base extension technique (SNaPshot). The serum PTEN levels were determined using an enzyme-linked immunosorbent assay (ELISA) kit. RESULTS: The rs1903858 AG, GG genotypes, and G allele were associated with a higher risk of NSCLC (odds ratio (OR) =2.079, 95% confidence interval (CI) = 1.087-3.974, P = .027; OR = 1.897, 95%CI = 1.053-3.419, P = .033; OR = 1.505, 95%CI = 1.065-2.126, P = .020). Stratified analysis reveal that the rs1903858 GG genotype and G allele were associated with an increased risk of squamous cell carcinoma (SCC) (OR = 3.226, 95%CI = 1.075-9.678, P = .037; OR = 1.873, 95%CI = 1.092-3.212, P = .023). Among smokers, the rs1903858 G allele carriers have an increased risk of NSCLC (OR = 1.916, 95%CI = 1.023-3.589, P = .042), but a decreased risk of NSCLC was found with the AT haplotype. With respect to the serum PTEN levels, no significant difference was noted between NSCLCpatients and healthy controls in this study. CONCLUSIONS: The study indicated that the rs1903858 gene polymorphism is associated with increased risk of NSCLC, particularly in SCC and smoker, and the haplotype AT was a protective factor for NSCLC. The serum PTEN levels were not associated with NSCLC.
Authors: Lili Gong; Jeane M Govan; Elizabeth B Evans; Hui Dai; Edward Wang; Szu-Wei Lee; Hui-Kuan Lin; Alexander J Lazar; Gordon B Mills; Shiaw-Yih Lin Journal: Cell Cycle Date: 2015 Impact factor: 4.534
Authors: Yafang Li; Xiangjun Xiao; Younghun Han; Olga Gorlova; David Qian; Natasha Leighl; Jakob S Johansen; Matt Barnett; Chu Chen; Gary Goodman; Angela Cox; Fiona Taylor; Penella Woll; H-Erich Wichmann; Judith Manz; Thomas Muley; Angela Risch; Albert Rosenberger; Susanne M Arnold; Eric B Haura; Ciprian Bolca; Ivana Holcatova; Vladimir Janout; Milica Kontic; Jolanta Lissowska; Anush Mukeria; Simona Ognjanovic; Tadeusz M Orlowski; Ghislaine Scelo; Beata Swiatkowska; David Zaridze; Per Bakke; Vidar Skaug; Shanbeh Zienolddiny; Eric J Duell; Lesley M Butler; Richard Houlston; María Soler Artigas; Kjell Grankvist; Mikael Johansson; Frances A Shepherd; Michael W Marcus; Hans Brunnström; Jonas Manjer; Olle Melander; David C Muller; Kim Overvad; Antonia Trichopoulou; Rosario Tumino; Geoffrey Liu; Stig E Bojesen; Xifeng Wu; Loic Le Marchand; Demetrios Albanes; Heike Bickeböller; Melinda C Aldrich; William S Bush; Adonina Tardon; Gad Rennert; M Dawn Teare; John K Field; Lambertus A Kiemeney; Philip Lazarus; Aage Haugen; Stephen Lam; Matthew B Schabath; Angeline S Andrew; Pier Alberto Bertazzi; Angela C Pesatori; David C Christiani; Neil Caporaso; Mattias Johansson; James D McKay; Paul Brennan; Rayjean J Hung; Christopher I Amos Journal: Carcinogenesis Date: 2018-03-08 Impact factor: 4.944