Literature DB >> 32518624

Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging.

Stephen D Grant1, Gemma S Cairns1, Jordan Wistuba1, Brian R Patton1.   

Abstract

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200. Copyright:
© 2019 Grant SD et al.

Entities:  

Keywords:  3D Printing; Light Microscopy; Open Science; Super-resolution

Year:  2019        PMID： 32518624      PMCID： PMC7255852          DOI： 10.12688/f1000research.21294.1

Source DB:  PubMed          Journal:  F1000Res        ISSN： 2046-1402


  7 in total

1.  Fiji: an open-source platform for biological-image analysis.

Authors:  Johannes Schindelin; Ignacio Arganda-Carreras; Erwin Frise; Verena Kaynig; Mark Longair; Tobias Pietzsch; Stephan Preibisch; Curtis Rueden; Stephan Saalfeld; Benjamin Schmid; Jean-Yves Tinevez; Daniel James White; Volker Hartenstein; Kevin Eliceiri; Pavel Tomancak; Albert Cardona
Journal:  Nat Methods       Date:  2012-06-28       Impact factor: 28.547

2.  A one-piece 3D printed flexure translation stage for open-source microscopy.

Authors:  James P Sharkey; Darryl C W Foo; Alexandre Kabla; Jeremy J Baumberg; Richard W Bowman
Journal:  Rev Sci Instrum       Date:  2016-02       Impact factor: 1.523

3.  Measuring image resolution in optical nanoscopy.

Authors:  Robert P J Nieuwenhuizen; Keith A Lidke; Mark Bates; Daniela Leyton Puig; David Grünwald; Sjoerd Stallinga; Bernd Rieger
Journal:  Nat Methods       Date:  2013-04-28       Impact factor: 28.547

4.  Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy.

Authors:  Graeme E Johnstone; Gemma S Cairns; Brian R Patton
Journal:  R Soc Open Sci       Date:  2019-07-31       Impact factor: 2.963

5.  ImageJ2: ImageJ for the next generation of scientific image data.

Authors:  Curtis T Rueden; Johannes Schindelin; Mark C Hiner; Barry E DeZonia; Alison E Walter; Ellen T Arena; Kevin W Eliceiri
Journal:  BMC Bioinformatics       Date:  2017-11-29       Impact factor: 3.169

6.  SRRF: Universal live-cell super-resolution microscopy.

Authors:  Siân Culley; Kalina L Tosheva; Pedro Matos Pereira; Ricardo Henriques
Journal:  Int J Biochem Cell Biol       Date:  2018-05-28       Impact factor: 5.085

7.  Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations.

Authors:  Nils Gustafsson; Siân Culley; George Ashdown; Dylan M Owen; Pedro Matos Pereira; Ricardo Henriques
Journal:  Nat Commun       Date:  2016-08-12       Impact factor: 14.919
  7 in total
  3 in total

Review 1.  Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging.

Authors:  John S H Danial; Jeff Y L Lam; Yunzhao Wu; Matthew Woolley; Eleni Dimou; Matthew R Cheetham; Derya Emin; David Klenerman
Journal:  Nat Protoc       Date:  2022-08-24       Impact factor: 17.021

2.  Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders.

Authors:  Siobhan Goss; Carlos Barba Bazan; Kaitlyn Neuman; Christina Peng; Nola Begeja; Celeste Elisabeth Suart; Ray Truant
Journal:  PLoS One       Date:  2022-06-03       Impact factor: 3.752

Review 3.  Can super-resolution microscopy become a standard characterization technique for materials chemistry?

Authors:  Shikha Dhiman; Teodora Andrian; Beatriz Santiago Gonzalez; Marrit M E Tholen; Yuyang Wang; Lorenzo Albertazzi
Journal:  Chem Sci       Date:  2021-12-01       Impact factor: 9.825
  3 in total

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