Edward D Chan1, Deepshikha Verma2, Diane J Ordway2. 1. Department of Medicine, Rocky Mountain Regional Veterans Affairs Medical Center, Departments of Medicine and Academic Affairs, National Jewish Health, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Anschutz Medical Campus, Denver, Colorado. 2. Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado.
Abstract
This manuscript describes the infection of mice and guinea pigs with mycobacteria via various routes, as well as necropsy methods for the determination of mycobacterial loads within target organs. Additionally, methods for cultivating mycobacteria and preparing stocks are described. The protocols outlined are primarily used for M. tuberculosis, but can also be used for the study of other non-tuberculosis mycobacterial species. A wide variety of animal models have been used to test new vaccines, drugs, and the impact of cigarette exposure.
This manuscript describes the infection of mice and guinea pigs with mycobacteria via various routes, as well as necropsy methods for the determination of mycobacterial loads within target organs. Additionally, methods for cultivating mycobacteria and preparing stocks are described. The protocols outlined are primarily used for M. tuberculosis, but can also be used for the study of other non-tuberculosis mycobacterial species. A wide variety of animal models have been used to test new vaccines, drugs, and the impact of cigarette exposure.
Authors: Hee-Jeong Yang; Decheng Wang; Xin Wen; Danielle M Weiner; Laura E Via Journal: Front Cell Infect Microbiol Date: 2021-03-16 Impact factor: 5.293
Authors: Bhagwati Khatri; James Keeble; Belinda Dagg; Daryan A Kaveh; Philip J Hogarth; Mei Mei Ho Journal: Sci Rep Date: 2021-12-02 Impact factor: 4.996