Alireza Tabibzadeh1, Farhad Zamani2, Azadeh Laali3, Maryam Esghaei1, Fahimeh Safarnezhad Tameshkel2, Hossein Keyvani4, Mahin Jamshidi Makiani3, Mahshid Panahi5, Nima Motamed6, Dhayaneethie Perumal7, Mahmoodreza Khoonsari2, Hossein Ajdarkosh2, Masuodreza Sohrabi2, Behrooz Ghanbari2, Shokoufeh Savaj8, Alireza Mosavi-Jarrahi9, Mohammad Hadi Karbalaie Niya10. 1. Department of Virology, Iran University of Medical Sciences, Tehran, Iran. 2. Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran. 3. Antimicrobial Resistant Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. 4. Department of Virology, Iran University of Medical Sciences, Tehran, Iran; Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran. 5. Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Pathology, Iran University of Medical Sciences, Tehran, Iran. 6. Department of Social Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. 7. Faculty of Science, Engineering and Computing, Kingston University, Kingston, United Kingdom. 8. Department of Nephrology, Firoozgar Hospital, Iran University of Medical Science, Tehran, Iran. 9. School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 10. Department of Virology, Iran University of Medical Sciences, Tehran, Iran; Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran. Electronic address: karbalai.mh@iums.ac.ir.
Abstract
BACKGROUND: The aim of the current study was to investigate and track the SARS-CoV-2 in Iranian Coronavirus Disease 2019 (COVID-19) patients using molecular and phylogenetic methods. METHODS: We enrolled seven confirmed cases of COVID-19 patients for the phylogenetic assessment of the SARS-CoV-2 in Iran. The nsp-2, nsp-12, and S genes were amplified using one-step RT-PCR and sequenced using Sanger sequencing method. Popular bioinformatics software were used for sequences alignment and analysis as well as phylogenetic construction. RESULTS: The mean age of the patients in the present study was 60.42 ± 9.94 years and 57.1% (4/7) were male. The results indicated high similarity between Iranian and Chinese strains. We could not find any particular polymorphisms in the assessed regions of the three genes. Phylogenetic trees by neighbor-joining and maximum likelihood method of nsp-2, nsp-12, and S genes showed that there are not any differences between Iranian isolates and those of other countries. CONCLUSION: As a preliminary phylogenetic study in Iranian SARS-CoV-2 isolates, we found that these isolates are closely related to the Chinese and reference sequences. Also, no sensible differences were observed between Iranian isolates and those of other countries. Further investigations are recommended using more comprehensive methods and larger sample sizes.
BACKGROUND: The aim of the current study was to investigate and track the SARS-CoV-2 in Iranian Coronavirus Disease 2019 (COVID-19) patients using molecular and phylogenetic methods. METHODS: We enrolled seven confirmed cases of COVID-19patients for the phylogenetic assessment of the SARS-CoV-2 in Iran. The nsp-2, nsp-12, and S genes were amplified using one-step RT-PCR and sequenced using Sanger sequencing method. Popular bioinformatics software were used for sequences alignment and analysis as well as phylogenetic construction. RESULTS: The mean age of the patients in the present study was 60.42 ± 9.94 years and 57.1% (4/7) were male. The results indicated high similarity between Iranian and Chinese strains. We could not find any particular polymorphisms in the assessed regions of the three genes. Phylogenetic trees by neighbor-joining and maximum likelihood method of nsp-2, nsp-12, and S genes showed that there are not any differences between Iranian isolates and those of other countries. CONCLUSION: As a preliminary phylogenetic study in Iranian SARS-CoV-2 isolates, we found that these isolates are closely related to the Chinese and reference sequences. Also, no sensible differences were observed between Iranian isolates and those of other countries. Further investigations are recommended using more comprehensive methods and larger sample sizes.
Authors: Juliana M Serpeloni; Quirino Alves Lima Neto; Léia Carolina Lucio; Anelisa Ramão; Jaqueline Carvalho de Oliveira; Daniela Fiori Gradia; Danielle Malheiros; Adriano Ferrasa; Rafael Marchi; David L A Figueiredo; Wilson A Silva; Enilze M S F Ribeiro; Ilce M S Cólus; Luciane R Cavalli Journal: Immunobiology Date: 2021-08-17 Impact factor: 3.152