Real-World Safety and Effectiveness of Dimethyl Fumarate in Hispanic or Latino Patients with Multiple Sclerosis: 3-Year Results from ESTEEM.

INTRODUCTION: Compared with the non-Hispanic/non-Latino population, Hispanic/Latino patients with multiple sclerosis (MS) are reported to exhibit greater disease severity. Geographical location and genetics play a role in differences observed across Hispanic/Latino subpopulations. We evaluated real-world safety and effectiveness of dimethyl fumarate (DMF) on MS disease activity in Hispanic/Latino patients.
METHODS: ESTEEM is an ongoing, 5-year, multinational, prospective study evaluating long-term safety and effectiveness of DMF in patients with MS. This interim analysis included patients newly prescribed DMF in routine practice at 394 sites globally.
RESULTS: Overall, 4986 non-Hispanic/non-Latino and 98 Hispanic/Latino patients were analyzed; median (range) follow-up was 18 (2-37) months. Unadjusted annualized relapse rates (ARRs) for 12 months before DMF initiation versus 36 months post DMF initiation, respectively, were: non-Hispanic/non-Latino patients, 0.82 (95% CI 0.80-0.84) versus 0.10 (95% CI 0.09-0.10), 88% lower ARR (P < 0.0001); Hispanic/Latino patients, 0.80 (95% CI 0.65-1.00) versus 0.09 (95% CI 0.06-0.14), 89% lower ARR (P < 0.0001). In total, 28 (29%) Hispanic/Latino patients reported adverse events leading to treatment discontinuation; gastrointestinal (GI) disorders (n = 10, 10%) were the most common, consistent with the non-Hispanic/non-Latino population (8%). Median lymphocyte counts decreased by approximately 24% in the first year (vs 36% decrease in non-Hispanic/non-Latino patients) then remained stable and above the lower limit of normal in most patients.
CONCLUSIONS: Relapse rates remained low in Hispanic/Latino patients, consistent with non-Hispanic/non-Latino patients. The safety profile of DMF in Hispanic/Latino patients was consistent with safety findings from the non-Hispanic/non-Latino ESTEEM population, demonstrating the real-world treatment benefit of DMF in the Hispanic/Latino patient cohort.

Key Summary Points

Introduction

Ethnic minority populations continue to be underrepresented in multiple sclerosis (MS) research in the real-world setting and in clinical trials. For example, of approximately 60,000 published articles on MS found through a PubMed literature review conducted in 2014 by Khan and colleagues [1], only 23 focused on Hispanic-American patient populations with MS. The underrepresentation of these populations leads to limited data on the effectiveness of treatments in these groups of patients and underscores the lack of an evidence-based approach used in treatment. Also, the limited studies and subgroup analyses that have been conducted have labeled these groups differently, as the nomenclature in the literature is inconsistent.

Dimethyl fumarate (DMF) and diroximel fumarate are oral, disease-modifying therapies approved for treating relapsing forms of MS. DMF and diroximel fumarate rapidly undergo esterase cleavage to monomethyl fumarate, a pharmacologically active metabolite [2, 3]. As of January 31, 2020, more than 445,000 patients have been treated with DMF worldwide, representing more than 875,000 patient-years of exposure. Of these, 6335 patients (14,241 patient-years) were from clinical trials. DMF has demonstrated strong, sustained efficacy and a favorable benefit–risk profile in patients with relapsing–remitting multiple sclerosis in the pivotal phase 3 studies DEFINE and CONFIRM, the ongoing open-label ENDORSE extension study, and real-world studies [4-6]. Although data from DEFINE and CONFIRM demonstrated a significant reduction in relapses in Hispanic patients, the sample size (n = 54) was too small to draw definitive conclusions regarding the effects of DMF in this subgroup [7]. ESTEEM (ClinicalTrials.gov identifier NCT02047097) is an ongoing, 5-year, multinational, prospective, noninterventional, phase 4 study evaluating the long-term safety and effectiveness of DMF in patients treated in real-world clinical practice. In this interim analysis, we evaluated the safety and effectiveness of DMF on MS disease activity in non-Hispanic/non-Latino patients and Hispanic/Latino patients, and in a cohort of Hispanic/Latino patients who switched from another therapy to DMF (received prior interferon [IFN] or glatiramer acetate [GA] at any time following diagnosis of MS).

Methods

Study Design and Patients

This interim analysis of the ESTEEM study data (data cutoff April 3, 2019) included patients with MS who had been newly prescribed DMF in routine practice at 394 sites globally. Hispanic/Latino patients were those who self-reported their ethnicity as “Hispanic or Latino”. Non-Hispanic/non-Latino patients were defined as patients who did not select “Hispanic or Latino” as their ethnicity. Of note, race was captured separately and was also self-reported; among the subgroup of patients who identified their ethnicity as Hispanic or Latino, most identified their race as “White”.

Patients under the age of 12 years are excluded from the ESTEEM study given the challenges of enrolling a sufficient number of patients, as less than 1% of the MS population are 11 years of age or under [8]. Patients aged between 12 and less than 18 years are also excluded where enrollment of pediatric patients is prohibited. In all, 5000 patients will be monitored for 5 years via routine clinical visits. The primary objective of the ESTEEM study was to determine the incidence, type, and pattern of serious adverse events (SAEs), including serious infections, and of AEs leading to treatment discontinuation. Magnetic resonance imaging data that would enable the characterization of disease severity at baseline were unavailable. A secondary objective of the interim analysis reported here was to consider the effectiveness of DMF on MS disease activity in non-Hispanic/non-Latino patients, Hispanic/Latino patients, and a subgroup of Hispanic/Latino patients who switched from another therapy to DMF (received prior IFN/GA at any time from diagnosis). This was achieved through the assessment of annualized relapse rate (ARR), the proportion of patients with a relapse, and the distribution of the number of relapses. The incidence of treatment discontinuation was also assessed.

The study was conducted in accordance with relevant US federal regulations, the Declaration of Helsinki, and the International Council on Harmonisation Guideline for Good Clinical Practice. Approvals were granted by relevant institutional ethics committees for study protocol and amendments, and written assent and consent forms were obtained from each patient and his or her parent or legal guardian. ESTEEM was registered at ClinicalTrials.gov (NCT02047097).

Statistical Analysis

ARRs were obtained by fitting a repeated-measure negative binomial model (ARR was defined as the total number of relapses during the study for all patients divided by the total number of patient-years in the study adjusted for appropriate prognostic factors, and time-to-event endpoints analyzed using Kaplan–Meier estimates). Descriptive summary statistics were calculated.

Results

Study Population

In total, 4986 non-Hispanic/non-Latino and 98 Hispanic/Latino patients, including 64 Hispanic/Latino patients who switched to DMF from IFN/GA, received at least one dose of DMF, and were included in this analysis. Baseline characteristics are shown in Table 1; the majority of non-Hispanic/non-Latino patients were of European background (56%), whereas 98% of Hispanic/Latino patients were from the USA (including Puerto Rico). The mean (SD) age of Hispanic/Latino patients was 40 (11.6) years, and 79% of these patients were female. No patients in the Hispanic/Latino patient group were under 18 years of age (non-Hispanic/non-Latino population, n = 10; Hispanic/Latino population, n = 0 [minimum age, 19 years]). Although the percentage of patients over 40 years of age was greater in the non-Hispanic/non-Latino cohort (49%; 2465/4986) than the Hispanic/Latino population (48%; 47/98), this difference was not of statistical significance.

Table 1

Patient baseline characteristics

n (%), unless otherwise specified	Non-Hispanic/Non-Latino patientsn = 4986	Hispanic/Latino patientsn = 98
Mean (SD) age, years	40 (11.2)	40 (11.6)
Age category, years
< 40	2520 (51)	51 (52)
≥ 40	2465 (49)	47 (48)
Sex, F	3681 (74)	77 (79)
Region
USA, including Puerto Rico	1301 (26)	96 (98)
Western Europe, Canada, New Zealand, and Australia	2811 (56)	1 (1)
Eastern Europe plus India, Mexico, and Argentina	874 (18)	1 (1)
Median (range) age at MS diagnosis, years	34 (9–83)	34 (17–67)
Median (range) duration between most recent relapse and enrollment, months	5.8 (0–325)	6.8 (0–410)
Median (range) number of relapses within past 12 months	1 (0–6)	1 (0–4)
Mean (SD) DMF treatment duration, months	21.5 (16.3)	19.0 (15.0)
Patients with minimum 1-year follow-up	3179 (64)	59 (60)
Patients with minimum 2-year follow-up	1915 (38)	32 (33)
Median (range) duration in ESTEEM, months	24.4 (0–63)	23.8 (1–55)
Mean (SD) EDSS scoreb	2.10 (1.48)	2.46 (2.35)
Prior MS treatment	3181 (64)	67 (68)
Glatiramer acetate	1255 (40)	30 (45)
Intramuscular IFNβ-1a	958 (30)	28 (42)
Subcutaneous IFNβ-1a	1511 (48)	33 (49)
IFNβ-1b	191 (6)	4 (6)
Fingolimod	284 (9)	9 (13)
Teriflunomide	218 (7)	3 (5)
Tobacco use	2803	68
Current user	719 (26)	7 (10)
Non-tobacco user	2084 (74)	61 (90)

DMF dimethyl fumarate, EDSS Expanded Disability Status Scale, IFN interferon, MS multiple sclerosis

aAge = (year diagnosed or year of first MS symptom − year of birth + 1)

bFor patients with available data from EDSS assessment at enrollment. Non-Hispanic/Non-Latino patients, n = 2798; Hispanic/Latino patients, n = 23

ARR and Relapses

The mean (SD) duration between most recent relapse and enrollment was 21.0 (35.54) months for the non-Hispanic/non-Latino population and 31.8 (63.92) months for the Hispanic/Latino population. The mean (SD) number of relapses in the prior 3 years was 1.4 (1.32) for the non-Hispanic/non-Latino population and 1.4 (2.06) for the Hispanic/Latino population, indicating no difference between the two groups. Among non-Hispanic/non-Latino patients, ARR 12 months before DMF initiation was 0.82 (95% CI 0.80–0.84) and after 36 months it was 0.10 (95% CI 0.09–0.10; P < 0.0001; Fig. 1). Among Hispanic/Latino patients, ARR 12 months before DMF initiation was 0.80 (95% CI 0.65–1.00) and after 36 months it was 0.09 (95% CI 0.06–0.14; P < 0.0001; Fig. 1).

Fig. 1

ARR in the 12 months before and 36 months after DMF initiation for the non-Hispanic/non-Latino, Hispanic/Latino, and Hispanic/Latino IFN/GA switch subgroups. ARR annualized relapse rate, DMF dimethyl fumarate, GA glatiramer acetate, IFN interferon. aPatients with missing relapse-rate data 12 months before DMF initiation were excluded from the ARR analysis. ARRs were obtained by fitting a repeated-measure negative binomial model

For the Hispanic/Latino IFN/GA switch population, ARR 12 months before switching was 0.73 (95% CI 0.54–1.00) versus 0.10 (95% CI 0.05–0.20) after 36 months (P < 0.0001, Fig. 1). The estimated proportion of relapse-free patients at 36 months was similar (80–88%) in non-Hispanic/non-Latino patients, Hispanic/Latino patients, and Hispanic/Latino patients who switched therapy (Table 2).

Table 2

Proportion of relapse-free patients in the 36 months after DMF initiation

	Non-Hispanic/Non-Latino patientsn = 4986	Hispanic/Latino patientsn = 98	Hispanic/Latino IFN/GA switch patientsn = 64
Proportion of patients without MS relapsea	80	84	88

Values are given as percentage

DMF dimethyl fumarate, GA glatiramer acetate, IFN interferon, MS multiple sclerosis

aKaplan-Meier analyses

Safety and Discontinuations

A total of 937 (19%) non-Hispanic/non-Latino patients and 28 (29%) Hispanic/Latino patients reported any AE leading to treatment discontinuation (Table 3). Gastrointestinal (GI) disorders were the most commonly cited (non-Hispanic/non-Latino patients, n = 385 [8%]; Hispanic/Latino patients, n = 10 [10%]), the majority of these GI-related discontinuations occurring within the first month of DMF treatment (non-Hispanic/non-Latino population, n = 271/385 [70%]; Hispanic/Latino, n = 8/10 [80%]). SAEs were reported in 239/4986 (5%) non-Hispanic/non-Latino patients and 6/98 (6%) Hispanic/Latino patients.

Table 3

Most common AEs leading to DMF discontinuation in the overall ESTEEM non-Hispanic/non-Latino and Hispanic/Latino patient population

Category, n (%)a	Non-Hispanic/Non-Latino patientsn = 4986	Hispanic/Latino patientsn = 98
Any AE leading to treatment discontinuation	937 (19)	28 (29)
GI disorders	385 (8)	10 (10)
Diarrhea	115 (2)	3 (3)
Nausea	105 (2)	2 (2)
Abdominal pain	67 (1)	2 (2)
Vomiting	63 (1)	1 (1)
Skin and subcutaneous tissue disorders	103 (2)	7 (7)
Rash	18 (< 1)	2 (2)
Rash, generalized	5 (< 1)	2 (2)
Infections	30 (< 1)	3 (3)
Investigations	93 (2)	2 (2)
Lymphocyte count decreased	52 (1)	2 (2)
Nervous system disorders	100 (2)	3 (3)
Blood and lymphatic system disorders	142 (3)	3 (3)
General disorders and administration site conditions	67 (1)	2 (2)

Values are given as number (percentage)

AE adverse event, DMF dimethyl fumarate, GI gastrointestinal

aAEs occurring in > 1 patient in the Hispanic/Latino subgroup. AEs were coded using the Medical Dictionary for Regulatory Activities (MedDRA) System Organ Class (SOC) and Preferred Term (PT). Percentages were calculated on the basis of total number of patients in each group. A patient was counted only once in each MedDRA SOC or PT

Absolute Lymphocyte Counts

Mean lymphocyte change over time for a duration of 36 months is shown in Fig. 2. In Hispanic/Latino patients, among patients with available lymphocyte data (n = 31), median absolute lymphocyte count (ALC) decreased 24% from baseline (1.96 × 109/L) to month 12 (1.41 × 109/L) and then remained stable. Median lymphocyte count remained above the lower limit of normal (0.91 × 109/L); however, the number of patients with median lymphocyte count by month 36 was limited (n = 9). In all other patients with available lymphocyte data (n = 2380), median ALC decreased 36% from baseline (1.97 × 109/L) to month 12 (1.22 × 109/L) and then remained stable.

Fig. 2

Median absolute lymphocyte count over time in Hispanic/Latino patients. LLN lower limit of normal (0.91 × 109/L). an number of patients with available data from absolute lymphocyte count assessment at the specified time point

Discussion

We report real-world safety and effectiveness of DMF on MS disease activity in non-Hispanic/non-Latino and Hispanic/Latino patients, including those Hispanic/Latino patients who also switched therapy to DMF from prior IFN/GA. Compared with the 12 months before DMF initiation, ARR was significantly lower in the 36 months after DMF initiation in the overall Hispanic/Latino cohort, and in Hispanic/Latino patients who switched from prior IFN/GA. These results are consistent with ARRs reported in the non-Hispanic/non-Latino population. No unexpected safety findings were observed in the Hispanic/Latino population, with AE and SAE profiles similar to those seen in non-Hispanic/non-Latino patients. Less than one-third of Hispanic/Latino patients reported AEs leading to treatment discontinuation, with GI disorders being the most commonly reported; this is very similar to reports from the non-Hispanic/non-Latino population (8%). It is not possible to draw a conclusion about AEs leading to treatment discontinuation owing to the small sample size; however, these results warrant further investigation with continued follow-up or additional studies.

The majority (80%) of GI disorders for the Hispanic/Latino population occurred within the first month of treatment. In this study, we observed a median 24% reduction in absolute lymphocyte count over the first year of DMF treatment in Hispanic/Latino patients, and absolute lymphocyte count remained above the lower limit of normal in all Hispanic/Latino patients, even at month 36 (n = 9). Tolerability and effectiveness of treatment with DMF across different racial groups may vary because clinically important differences in the course of MS and response to therapies exist among patients of different racial and ethnic backgrounds [9-13]. For example, Hispanic-American patients with MS may be more likely to experience optic neuritis, transverse myelitis, and low levels of vitamin D—conditions that can be considered risk factors for MS [14, 15]. These patients also tend to be of a younger age at disease onset and have a more aggressive disease course with more rapid disability progression compared with white patients with MS [9]. Our study provides evidence that, in this small cohort of Hispanic/Latino patients, DMF is tolerated and effective in routine clinical practice.

One of the primary strengths of the ESTEEM study is its design, which enables prospective observation of patients in routine clinical practice over time. In this interim analysis, DMF outcomes were analyzed in the largest Hispanic/Latino cohort used to date. We have reported on outcomes over the longest follow-up duration compared with previous studies. Potential differences in disease pathophysiology among ethnic groups merit further study and may have implications for the treatment of patients with MS. To enable more comprehensive and accurate data to be obtained about MS and its treatment in underserved populations, it is important that health care providers discuss participation in clinical trials with all patients, with the aim of increasing patient participation and subsequently the generalizability of clinical trial findings.

Study limitations include the observational open-label design, the lack of magnetic resonance imaging baseline data to accurately characterize the disease severity of this population, and the short follow-up duration. Nevertheless, this prospective study provides preliminary data that suggest that DMF outcomes in this Hispanic/Latino cohort are similar to those that have been observed in the non-Hispanic/non-Latino population, and could be as effective.

Another limitation of this study is that most of the patients in this subgroup were from the USA and therefore may not be representative of a global MS population. Stratified analyses based on ethnicity are not available for patients from Europe because of differences in data collection and data privacy regulations.

In conclusion, these safety and effectiveness analyses demonstrate the real-world treatment benefit of DMF in Hispanic/Latino patients, consistent with findings in the overall ESTEEM population. Similar findings also were observed in a subgroup analysis of African-American patients [16]. The ESTEEM study is ongoing; therefore, further insights into the safety and effectiveness of DMF in different ethnic groups may be obtained from the final 5-year study results.

Why carry out this study?

Delayed-release dimethyl fumarate (DMF) has demonstrated clinically meaningful, sustained efficacy and a favorable benefit–risk profile in patients with relapsing forms of multiple sclerosis (MS)

ESTEEM is an ongoing, 5-year, multinational, prospective study evaluating long-term safety and effectiveness of DMF newly prescribed to patients with MS

Geographical location and genetics play a role in differences observed across Hispanic subpopulations

In this interim analysis of ESTEEM, we evaluated real-world safety and effectiveness of DMF in the largest subgroup of DMF-treated Hispanic patients studied to date

What was learned from the study?

The safety profile and the effectiveness of DMF on relapse rates in Hispanic patients were consistent with findings observed from the non-Hispanic population

These analyses demonstrate the real-world treatment benefit of DMF in Hispanic patients, consistent with findings in the overall ESTEEM population