Fadime Aydoğan 1 , Şura Baykan 1 , Bilge Debeleç Bütüner 2 Show Affiliations »
Abstract
OBJECTIVES: This was a phytochemical study of endemic Ferula tenuissima roots and determined the cytotoxic activity of pure compounds on PC-3. MATERIALS AND METHODS: Air-dried and powdered roots of F. tenuissima (1 kg) were extracted consecutively with n-hexane, chloroform (CHCl3), and methanol (MeOH) (3×2 L, each) by sonication at 30°C for 24 h. The extracts were then filtered. The solvents were separately evaporated under reduced pressure to dryness. The compounds were isolated by chromatographic methods and their structures were determined by spectral methods (1D and 2D NMR and LC-MS). The compounds were tested for their cytotoxic activities versus the PC-3 cell line by WST assay. RESULTS: A phytochemical investigation of the dried roots of endemic F. tenuissima was performed and three sesquiterpene esters were isolated. The daucane-type sesquiterpenes teferidin, ferutinin, and elaeochytrin-A were identified. In the bioactivity study, ferutinin exhibited the highest cytotoxic activity, with an IC50 value of 19.7 μM. CONCLUSION: The results indicate that the main compounds of F. tenuissima roots are daucane sesquiterpenes and ferutinin has a potential effect on PC-3 cells. ©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.
OBJECTIVES: This was a phytochemical study of endemic Ferula tenuissima roots and determined the cytotoxic activity of pure compounds on PC-3. MATERIALS AND METHODS: Air-dried and powdered roots of F. tenuissima (1 kg) were extracted consecutively with n-hexane, chloroform (CHCl3), and methanol (MeOH) (3×2 L, each) by sonication at 30°C for 24 h. The extracts were then filtered. The solvents were separately evaporated under reduced pressure to dryness. The compounds were isolated by chromatographic methods and their structures were determined by spectral methods (1D and 2D NMR and LC-MS). The compounds were tested for their cytotoxic activities versus the PC-3 cell line by WST assay. RESULTS: A phytochemical investigation of the dried roots of endemic F. tenuissima was performed and three sesquiterpene esters were isolated. The daucane-type sesquiterpenes teferidin, ferutinin, and elaeochytrin-A were identified. In the bioactivity study, ferutinin exhibited the highest cytotoxic activity, with an IC50 value of 19.7 μM. CONCLUSION: The results indicate that the main compounds of F. tenuissima roots are daucane sesquiterpenes and ferutinin has a potential effect on PC-3 cells. ©Copyright 2019 Turk J Pharm Sci, Published by Galenos Publishing House.
Entities: Chemical
Keywords:
Ferula tenuissima; cytotoxicity; daucane sesquiterpene esters; prostate cancer
Year: 2019
PMID： 32454752 PMCID： PMC7227880 DOI： 10.4274/tjps.galenos.2018.23356
Source DB: PubMed Journal: Turk J Pharm Sci ISSN： 1304-530X