Xin Wen1, Huibin Lin2, Yilin Ren3, Can Li4, Chengjia Zhang1, Xin Song5, Jianqun Lin1, Jianqiang Lin6. 1. State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, China. 2. Shandong Academy of Chinese Medicine, Jinan, China. 3. Qingdao Longding Biotech Limited Company, Qingdao, China. 4. School of Biological Engineering, Qilu University of Technology, Jinan, China. 5. State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, China. songx@sdu.edu.cn. 6. State Key Laboratory of Microbial Technology, Shandong University (Qingdao), Qingdao, China. jianqianglin@sdu.edu.cn.
Abstract
OBJECTIVE: To develop a method combining enzymatic catalysis and resting-cell biotransformation to produce allitol from low cost substrate D-glucose. RESULTS: The recombinant E. coli expressing D-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) for allitol production from D-fructose was constructed. The optimizations of the cell catalytic conditions and the cell cultivation conditions were made. Then, 63.4 g allitol L-1 was obtained from 100 g D-fructose L-1 in 4 h catalyzed by the recombinant E. coli cells. In order to decrease the substrate cost, D-glucose was used as the substrate instead of D-fructose and immobilized glucose isomerase was used to convert D-glucose into D-fructose. In order to simplify allitol production process from D-glucose, one-pot reaction using the mixed catalysts was used and the reaction conditions were optimized. Finally, 12.7 g allitol L-1 was obtained from 50 g D-glucose L-1 catalyzed by the mixed catalysts of immobilized glucose isomerase and the recombinant E. coli cells. CONCLUSIONS: Allitol can be efficiently produced from low cost substrate D-glucose by using the method combining enzymatic catalysis and resting-cell biotransformation, which is the first report.
OBJECTIVE: To develop a method combining enzymatic catalysis and resting-cell biotransformation to produce allitol from low cost substrate D-glucose. RESULTS: The recombinant E. coli expressing D-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) for allitol production from D-fructose was constructed. The optimizations of the cell catalytic conditions and the cell cultivation conditions were made. Then, 63.4 g allitol L-1 was obtained from 100 g D-fructose L-1 in 4 h catalyzed by the recombinant E. coli cells. In order to decrease the substrate cost, D-glucose was used as the substrate instead of D-fructose and immobilized glucose isomerase was used to convert D-glucose into D-fructose. In order to simplify allitol production process from D-glucose, one-pot reaction using the mixed catalysts was used and the reaction conditions were optimized. Finally, 12.7 g allitol L-1 was obtained from 50 g D-glucose L-1 catalyzed by the mixed catalysts of immobilized glucose isomerase and the recombinant E. coli cells. CONCLUSIONS:Allitol can be efficiently produced from low cost substrate D-glucose by using the method combining enzymatic catalysis and resting-cell biotransformation, which is the first report.