Literature DB >> 32435071

The Spectrophotometric Characteristic of Immunoglobulin Conjugates for Diagnostics of Causative Agents of Especially Dangerous Infections.

A N Spitsyn1, D V Utkin1, M N Kireev1, M V Ovchinnikova1, O S Kuznetsov1, P S Erokhin1, V I Kochubei2,3.   

Abstract

The possibility of characterizing fluorescent immunoglobulins using spectrophotometric analysis as a testing method is considered. The comparative analysis of optical properties of fluorescent immunoglobulin preparations and their components-immunoglobulins and fluorochrome-is carried out. The obtained results testify that the proposed methodological approach of optical detection of labeled immunoglobulin molecules can be promising for tests on obtaining conjugates used in immunological tests on revealing specific antigens of causative agents of especially dangerous infections. © Pleiades Publishing, Ltd. 2020.

Entities:  

Keywords:  conjugates; fluorescein isothiocyanate; immunoglobulins; spectrophotometry

Year:  2020        PMID： 32435071      PMCID： PMC7224094          DOI： 10.1134/S0030400X20030182

Source DB:  PubMed          Journal:  Opt Spectrosc        ISSN： 0030-400X            Impact factor:   0.891


  14 in total

1.  Fluorescent labeling of chitosan for use in non-invasive monitoring of degradation in tissue engineering.

Authors:  Cassilda Cunha-Reis; Alicia J El Haj; Xuebin Yang; Ying Yang
Journal:  J Tissue Eng Regen Med       Date:  2011-11-28       Impact factor: 3.963

2.  Synthesis and applications of Rhodamine derivatives as fluorescent probes.

Authors:  Mariana Beija; Carlos A M Afonso; José M G Martinho
Journal:  Chem Soc Rev       Date:  2009-04-27       Impact factor: 54.564

3.  Rapid batch method for the preparation of fluorescein isothiocyanate-conjugated antibody.

Authors:  J V Calcagno; M J Sweeney; H C Oels
Journal:  Infect Immun       Date:  1973-03       Impact factor: 3.441

4.  Origin of a fluorescence increase accompanying the limited proteolysis of fluorescein-labeled human prothrombin by Factor Xa.

Authors:  S A Morrison
Journal:  Int J Pept Protein Res       Date:  1984-10

5.  Texas Res-X and rhodamine Red-X, new derivatives of sulforhodamine 101 and lissamine rhodamine B with improved labeling and fluorescence properties.

Authors:  C Lefevre; H C Kang; R P Haugland; N Malekzadeh; S Arttamangkul; R P Haugland
Journal:  Bioconjug Chem       Date:  1996 Jul-Aug       Impact factor: 4.774

6.  A microwave-assisted fluorescent labeling method for the separation and detection of amphetamine-like designer drugs by capillary electrophoresis.

Authors:  Kuan-Fu Chen; Hsun Lee; Ju-Tsung Liu; Huan-An Lee; Cheng-Huang Lin
Journal:  Forensic Sci Int       Date:  2013-03-22       Impact factor: 2.395

7.  Rhodamine based sensor for naked-eye detection and live cell imaging of fluoride ions.

Authors:  Gandhi Sivaraman; Duraisamy Chellappa
Journal:  J Mater Chem B       Date:  2013-09-20       Impact factor: 6.331

Review 8.  Fluorescent sensing of fluoride in cellular system.

Authors:  Yang Jiao; Baocun Zhu; Jihua Chen; Xiaohong Duan
Journal:  Theranostics       Date:  2015-01-01       Impact factor: 11.556

9.  A rapid procedure for preparing fluorescein-labeled specific antibodies from whole antiserum: its use in analyzing cytoskeletal architecture.

Authors:  J C Talian; J B Olmsted; R D Goldman
Journal:  J Cell Biol       Date:  1983-10       Impact factor: 10.539

10.  Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.

Authors:  A H COONS; M H KAPLAN
Journal:  J Exp Med       Date:  1950-01-01       Impact factor: 14.307
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