Aslaug Drotningsvik1,2, Åge Oterhals3, Svein Are Mjøs4, Linn Anja Vikøren5, Ola Flesland2, Oddrun Anita Gudbrandsen6. 1. Dietary Protein Research Group, Department of Clinical Medicine, University of Bergen, Haukeland University Hospital, 5021, Bergen, Norway. 2. TripleNine Vedde AS, 6030, Langevåg, Norway. 3. Nofima, 5828, Bergen, Norway. 4. Department of Chemistry, University of Bergen, 5020, Bergen, Norway. 5. Department of Clinical Science, University of Bergen, 5021, Bergen, Norway. 6. Dietary Protein Research Group, Department of Clinical Medicine, University of Bergen, Haukeland University Hospital, 5021, Bergen, Norway. oddrun.gudbrandsen@k1.uib.
Abstract
PURPOSE: To investigate the effects of diets containing intact or hydrolysed proteins from blue whiting (Micromesistius poutassou) on the development of high blood pressure and markers of kidney function in obese Zucker fa/fa rats which are prone to develop hypertension and renal failure. METHODS: Male rats were fed isocaloric diets containing either intact blue whiting whole meal (BW-WM), blue whiting protein hydrolysate prepared with Alcalase® (BW-HA) or blue whiting protein hydrolysate prepared with Protamex® (BW-HP) as 1/3 of total protein with the remaining 2/3 as casein, or casein as sole protein source (control group). Blood pressure was measured at Day 0 and Day 32. Rats were housed in metabolic cages for 24 h for collection of urine in week 4. After 5 weeks, rats were euthanized and blood was drawn from the heart. The renin and angiotensin-converting enzyme (ACE) inhibition capacities for casein and blue whiting proteins were measured in vitro. RESULTS: The blood pressure increase was lower in rats fed diets containing blue whiting proteins when compared to the control group, whereas markers of kidney function were similar between all groups. The three blue whiting proteins inhibited renin activity in vitro, whereas casein had no effect. The in vitro ACE inhibition was similar for casein, BW-WM and BW-HP proteins, whereas BW-HA protein was less potent. CONCLUSION: Blue whiting protein feeding attenuated the blood pressure increase in obese Zucker fa/fa rats, possibly mediated through the renin-angiotensin system and without affecting markers of kidney function.
PURPOSE: To investigate the effects of diets containing intact or hydrolysed proteins from blue whiting (Micromesistius poutassou) on the development of high blood pressure and markers of kidney function in obese Zucker fa/farats which are prone to develop hypertension and renal failure. METHODS: Male rats were fed isocaloric diets containing either intact blue whiting whole meal (BW-WM), blue whiting protein hydrolysate prepared with Alcalase® (BW-HA) or blue whiting protein hydrolysate prepared with Protamex® (BW-HP) as 1/3 of total protein with the remaining 2/3 as casein, or casein as sole protein source (control group). Blood pressure was measured at Day 0 and Day 32. Rats were housed in metabolic cages for 24 h for collection of urine in week 4. After 5 weeks, rats were euthanized and blood was drawn from the heart. The renin and angiotensin-converting enzyme (ACE) inhibition capacities for casein and blue whiting proteins were measured in vitro. RESULTS: The blood pressure increase was lower in rats fed diets containing blue whiting proteins when compared to the control group, whereas markers of kidney function were similar between all groups. The three blue whiting proteins inhibited renin activity in vitro, whereas casein had no effect. The in vitro ACE inhibition was similar for casein, BW-WM and BW-HP proteins, whereas BW-HA protein was less potent. CONCLUSION:Blue whiting protein feeding attenuated the blood pressure increase in obese Zucker fa/farats, possibly mediated through the renin-angiotensin system and without affecting markers of kidney function.
Entities:
Keywords:
Blood pressure; Fish proteins; Hypertension; Obesity; Rat; Zucker
Authors: August Hoel; Tarig Osman; Fredrik Hoel; Hassan Elsaid; Tony Chen; Lea Landolt; Janka Babickova; Karl Johan Tronstad; James B Lorens; Gro Gausdal; Hans-Peter Marti; Jessica Furriol Journal: J Cell Mol Med Date: 2021-07-05 Impact factor: 5.310