Complete genome sequence and antimicrobial activity of Bacillus velezensis JT3-1, a microbial germicide isolated from yak feces.

Bacillus velezensis JT3-1 was isolated from feces of the domestic yak (Bos grunniens) in Gansu province of China. Aim to know whether B. velezensis JT3-1 has the potency to be developed as a probiotic bacterium, works on the complete genome sequence, antimicrobial activity, growth performance in calves, and treatment effect on calf diarrhea of B. velezensis JT3-1 were carried out. The results showed that the complete genome of B. velezensis JT3-1 contains one gapless circular chromosome which is 3,929,799 bp, and has 3761 protein-encoding genes with an average GC content of 46.50%. From the antimicrobial activity results, B. velezensis JT3-1 has shown strong antagonistic activities against Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, Mannheimia haemolytica, Staphylococcus hominis, Clostridium perfringens, and Mycoplasma bovis. Compared with the control group, the average weight of the experiment animals from Bv1 group and Bv2 group which were supplemented with B. velezensis JT3-1 was significantly increased (P < 0.05). Meanwhile, the Bv1 and Bv2 supplement significantly improved the level of IgA, IgG, IgM, and IFN-γ in calves as compared with the controls (P < 0.05), but the IL-2 level was not obviously changed between the three groups. In addition, B. velezensis JT3-1 showed a good effect against diarrhea, as the cure rate reached 95.0% (171/180) in newborn calves (Angus cattle) in Xinjiang, and 100.0% (149/149) in yak calves in Qinghai, respectively. Our study will lay a good foundation for the elucidation of the molecular mechanisms of its antimicrobial activity, and supports the hypothesis that JT3-1 has the potential to be developed as a probiotic bacterium in cattle. © King Abdulaziz City for Science and Technology 2020.