| Literature DB >> 32398372 |
Shayne D Wierbowski1,2, Tommy V Vo2, Pascal Falter-Braun3,4, Timothy O Jobe5, Lars H Kruse6, Xiaomu Wei1, Jin Liang2, Michael J Meyer1,2, Nurten Akturk2, Christen A Rivera-Erick2, Nicolas A Cordero2, Mauricio I Paramo2,7, Elnur E Shayhidin2, Marta Bertolotti2, Nathaniel D Tippens1,2, Kazi Akther8, Rita Sharma9, Yuichi Katayose10, Kourosh Salehi-Ashtiani11,12,13,14, Tong Hao12,13, Pamela C Ronald15,16,17, Joseph R Ecker18,19, Peter A Schweitzer20, Shoshi Kikuchi21, Hiroshi Mizuno22, David E Hill12,13, Marc Vidal12,13, Gaurav D Moghe6, Susan R McCouch23, Haiyuan Yu24,2.
Abstract
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.Entities:
Keywords: ORFeome; networks; next-generation sequencing; protein–protein interaction; rice
Year: 2020 PMID： 32398372 PMCID： PMC7260977 DOI： 10.1073/pnas.1918068117
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205