Ankita Thawani1,2,3, Nabilah H Sammudin1,2, Hannah S Reygaerts1, Alexis N Wozniak1, Vidhya Munnamalai4, Richard J Kuhn1,3, Donna M Fekete1,2,3,5. 1. Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA. 2. Purdue Institute of Integrative Neuroscience, Purdue University, West Lafayette, Indiana, USA. 3. Purdue Institute of Inflammation Immunology and Infectious Disease, Purdue University, West Lafayette, Indiana, USA. 4. The Jackson Laboratory, Bar Harbor, Maine, USA. 5. Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana, USA.
Abstract
BACKGROUND: Sensorineural hearing loss is an understudied consequence of congenital Zika syndrome, and balance disorders are essentially unreported to date. Also lacking is information about the susceptibility and the pathogenesis of the developing inner ear following Zika virus (ZIKV) exposure. To address this, ZIKV was delivered directly into the otic cup/otocyst of chicken embryos and infection of inner ear tissues was evaluated using immunohistochemistry. RESULTS: After injections on embryonic days 2 to 5, ZIKV infection was observed in 90% of the samples harvested 2 to 8 days later; however, the degree of infection was highly variable across individuals. ZIKV was detected in all regions of the inner ear, associated ganglia, and in the surrounding periotic mesenchyme. Detection of virus peaked earlier in the ganglion and vestibular compartments, and later in the cochlea. ZIKV infection increased cell death robustly in the auditory ganglion, and modestly in the auditory sensory organ. Macrophage accumulation was found to overlap with dense viral infection in some tissues. Additionally, dysmorphogenesis of the semicircular canals and ganglion was observed for a subset of injection conditions. CONCLUSIONS: This article presents evidence of direct ZIKV infection of developing inner ear epithelium and shows previously unknown inner ear dysmorphogenesis phenotypes.
BACKGROUND:Sensorineural hearing loss is an understudied consequence of congenital Zika syndrome, and balance disorders are essentially unreported to date. Also lacking is information about the susceptibility and the pathogenesis of the developing inner ear following Zika virus (ZIKV) exposure. To address this, ZIKV was delivered directly into the otic cup/otocyst of chicken embryos and infection of inner ear tissues was evaluated using immunohistochemistry. RESULTS: After injections on embryonic days 2 to 5, ZIKVinfection was observed in 90% of the samples harvested 2 to 8 days later; however, the degree of infection was highly variable across individuals. ZIKV was detected in all regions of the inner ear, associated ganglia, and in the surrounding periotic mesenchyme. Detection of virus peaked earlier in the ganglion and vestibular compartments, and later in the cochlea. ZIKVinfection increased cell death robustly in the auditory ganglion, and modestly in the auditory sensory organ. Macrophage accumulation was found to overlap with dense viral infection in some tissues. Additionally, dysmorphogenesis of the semicircular canals and ganglion was observed for a subset of injection conditions. CONCLUSIONS: This article presents evidence of direct ZIKVinfection of developing inner ear epithelium and shows previously unknown inner ear dysmorphogenesis phenotypes.
Authors: Allison M Nishitani; Sho Ohta; Andrea R Yung; Tony Del Rio; Michael I Gordon; Victoria E Abraira; Evelyn C Avilés; Gary C Schoenwolf; Donna M Fekete; Lisa V Goodrich Journal: Development Date: 2017-08-29 Impact factor: 6.868
Authors: Camila V Ventura; Mauricio Maia; Bruna V Ventura; Vanessa Van Der Linden; Eveline B Araújo; Regina C Ramos; Maria Angela W Rocha; Maria Durce C G Carvalho; Rubens Belfort; Liana O Ventura Journal: Arq Bras Oftalmol Date: 2016-02 Impact factor: 0.872
Authors: Maria Helena de Magalhães Barbosa; Maria Clara de Magalhães-Barbosa; Jaqueline Rodrigues Robaina; Arnaldo Prata-Barbosa; Marco Antonio de Melo Tavares de Lima; Antonio José Ledo Alves da Cunha Journal: Braz J Otorhinolaryngol Date: 2019-06-18
Authors: Vidhya Munnamalai; Nabilah H Sammudin; Caryl A Young; Ankita Thawani; Richard J Kuhn; Donna M Fekete Journal: Viruses Date: 2021-09-14 Impact factor: 5.048