Paulina Borecka1, Katarzyna Ratajczak-Wielgomas2, Rafal Ciaputa3, Małgorzata Kandefer-Gola3, Izabela Janus3, Aleksandra Piotrowska2, Alicja Kmiecik2, Marzena Podhorska-Okolów4, Piotr Dzięgiel2,5, Marcin Nowak3. 1. Department of Pathology, Division of Pathomorphology and Forensic Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland Paulina.borecka@upwr.edu.pl. 2. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland. 3. Department of Pathology, Division of Pathomorphology and Forensic Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland. 4. Division of Ultrastructure Research, Wroclaw Medical University, Wroclaw, Poland. 5. Department of Physiotherapy, University School of Physical Education in Wroclaw, Wroclaw, Poland.
Abstract
BACKGROUND/AIM: Mammary neoplasms, like breast neoplasms in women, are one of the most common tumours in female dogs. Cancer-associated fibroblasts (CAFs) found in the tumour stroma play a role in angiogenesis and increase cell migration, contributing to tumour growth and progression, as well as metastasis. The aim of our work was to determine the level of periostin (POSTN) expression in CAFs in mammary tumours of female dogs. MATERIALS AND METHODS: The research material consisted of 77 carcinomas and 24 adenomas of the mammary ridge in female dogs. Immunohistochemistry tests were performed using antibodies directed against the antigens POSTN, Ki-67, ERB-B2 receptor tyrosine kinase 2 (HER2), vimentin, and alpha smooth muscle actin (αSMA). Expression of POSTN at the mRNA level was determined using real-time polymerase chain reaction methods in 20 cases of mammary neoplasms. RESULTS: Expression of POSTN in CAFs was observed in 92% of mammary cancer samples and in 25% of mammary adenoma samples in female dogs. A statistically significant increase in POSNT expression in CAFs was found in the carcinomas compared with mammary adenomas in female dogs. Expression of POSTN in CAFs in mammary carcinomas in female dogs positively correlated with the histological malignancy grade of tumours and the expression of Ki-67 proliferative antigen. CONCLUSION: Our results suggest a role of POSTN on the pathogenesis of mammary tumours in female dogs. Moreover, POSTN may prove to be a useful marker in the evaluation of cancerous stroma of mammary tumours in female dogs, and may have prognostic significance. Copyright
BACKGROUND/AIM: Mammary neoplasms, like breast neoplasms in women, are one of the most common tumours in female dogs. Cancer-associated fibroblasts (CAFs) found in the tumour stroma play a role in angiogenesis and increase cell migration, contributing to tumour growth and progression, as well as metastasis. The aim of our work was to determine the level of periostin (POSTN) expression in CAFs in mammary tumours of female dogs. MATERIALS AND METHODS: The research material consisted of 77 carcinomas and 24 adenomas of the mammary ridge in female dogs. Immunohistochemistry tests were performed using antibodies directed against the antigens POSTN, Ki-67, ERB-B2 receptor tyrosine kinase 2 (HER2), vimentin, and alpha smooth muscle actin (αSMA). Expression of POSTN at the mRNA level was determined using real-time polymerase chain reaction methods in 20 cases of mammary neoplasms. RESULTS: Expression of POSTN in CAFs was observed in 92% of mammary cancer samples and in 25% of mammary adenoma samples in female dogs. A statistically significant increase in POSNT expression in CAFs was found in the carcinomas compared with mammary adenomas in female dogs. Expression of POSTN in CAFs in mammary carcinomas in female dogs positively correlated with the histological malignancy grade of tumours and the expression of Ki-67 proliferative antigen. CONCLUSION: Our results suggest a role of POSTN on the pathogenesis of mammary tumours in female dogs. Moreover, POSTN may prove to be a useful marker in the evaluation of cancerous stroma of mammary tumours in female dogs, and may have prognostic significance. Copyright
Authors: Bartosz Pula; Aleksandra Jethon; Aleksandra Piotrowska; Agnieszka Gomulkiewicz; Tomasz Owczarek; Jacek Calik; Andrzej Wojnar; Wojciech Witkiewicz; Janusz Rys; Maciej Ugorski; Piotr Dziegiel; Marzena Podhorska-Okolow Journal: Histopathology Date: 2011-12 Impact factor: 5.087
Authors: Andreia A Santos; Célia C Lopes; Jorge R Ribeiro; Liliana R Martins; Joana C Santos; Irina F Amorim; Fátima Gärtner; Augusto J Matos Journal: BMC Vet Res Date: 2013-01-04 Impact factor: 2.741
Authors: Paulina Borecka; Rafal Ciaputa; Izabela Janus; Joanna Bubak; Aleksandra Piotrowska; Katarzyna Ratajczak-Wielgomas; Marzenna Podhorska-OkolÓw; Piotr DziĘgiel; Marcin Nowak Journal: In Vivo Date: 2020 Nov-Dec Impact factor: 2.155