| Literature DB >> 32353253 |
Xin Gong1, Diego Mendoza-Halliday2, Jonathan T Ting3, Tobias Kaiser2, Xuyun Sun4, André M Bastos5, Ralf D Wimmer2, Baolin Guo6, Qian Chen2, Yang Zhou2, Maxwell Pruner2, Carolyn W-H Wu2, Demian Park2, Karl Deisseroth7, Boaz Barak2, Edward S Boyden2, Earl K Miller5, Michael M Halassa8, Zhanyan Fu6, Guoqiang Bi9, Robert Desimone10, Guoping Feng11.
Abstract
Optogenetics is among the most widely employed techniques to manipulate neuronal activity. However, a major drawback is the need for invasive implantation of optical fibers. To develop a minimally invasive optogenetic method that overcomes this challenge, we engineered a new step-function opsin with ultra-high light sensitivity (SOUL). We show that SOUL can activate neurons located in deep mouse brain regions via transcranial optical stimulation and elicit behavioral changes in SOUL knock-in mice. Moreover, SOUL can be used to modulate neuronal spiking and induce oscillations reversibly in macaque cortex via optical stimulation from outside the dura. By enabling external light delivery, our new opsin offers a minimally invasive tool for manipulating neuronal activity in rodent and primate models with fewer limitations on the depth and size of target brain regions and may further facilitate the development of minimally invasive optogenetic tools for the treatment of neurological disorders.Entities:
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Year: 2020 PMID： 32353253 PMCID： PMC7351618 DOI： 10.1016/j.neuron.2020.03.032
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173