μ-Thin-layer chromatography coupled with laser ablation-inductively coupled plasma-mass spectrometry using tin(II)-imprinted polymer nanoparticles as a stationary phase for speciation of tin.

A unique and novel μ-thin-layer chromatography method based on Sn(II) ion-imprinted polymer (Sn-IIP) for speciation of tin ion species in water and plasma samples is introduced for the first time. For this purpose, N-allylthiourea (NATU) and ethylene glycol dimethacrylate (EGDMA) were copolymerized in the presence of Sn(II). The obtained polymer particles were identified using multiple techniques like BET, FT-IR, XRD, and FESEM. The effects of different variables such as pH of the solution, mobile phase composition, and IIP per CaSO4 mass ratio on the separation efficiency were also evaluated. After completion of the separation process on the plate, its surface was scanned by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Under the established optimal condition, the detection limit, relative standard deviation (RSD) of responses, and linear dynamic range (LDR) of the method were obtained as 0.3 μg L-1, 3.5%, and 0.8-900 μg L-1 for Sn(II) and 0.4 μg L-1, 4%, and 1-740 μg L-1 for Sn(IV) assay, respectively. The developed method was finally applied to the speciation of tin in various water and plasma samples. Graphical abstract Schematic representation of μ-thin-layer chromatography method based on tin(II) ion-imprinted polymer (Sn-IIP) for speciation of tin ion species in water and plasma samples and scanned separated casts by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS).