Multifunctional laser speckle imaging.

We have developed a multi-functional laser speckle imaging system, which can be operated in both the surface illumination laser speckle contrast imaging (SI-LSCI) mode and the line scan laser speckle contrast imaging (LS-LSCI) mode. The system has been applied to imaging the chicken embryos to visualize both the blood flow and morphological details of the vasculature. The experimental results demonstrated that LS-LSCI is capable of detecting and quantifying blood flow in blood vessels smaller and deeper than those detectable by conventional SI-LSCI. Furthermore, the line scan mode is also capable of producing depth-resolved absorption-based morphological images of tissue, augmenting flow-based functional images.