Literature DB >> 323276

Use of the enzyme-linked immunosorbent assay (ELISA) and its microadaptation for the serodiagnosis of toxoplasmosis.

K W Walls, S L Bullock, D K English.   

Abstract

The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the microtitration method were used successfully. ELISA results are equivalent to those found in the indirect immunofluorescence test, yet the ELISA procedure is simpler and more rapid to perform.

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Year:  1977        PMID： 323276      PMCID： PMC274581          DOI： 10.1128/jcm.5.3.273-277.1977

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  New serological test for malaria antibodies.

Authors:  A Voller; G Huldt; C Thors; E Engvall
Journal:  Br Med J       Date:  1975-03-22

2.  Letter: Application of ELISA--enzyme linked immunosorbent assay--in the sero-diagnosis of amoebiasis.

Authors:  H J Bos; A A Eijk; P A Steerenberg
Journal:  Trans R Soc Trop Med Hyg       Date:  1975       Impact factor: 2.184

3.  [Immunoenzymologic diagnosis of parasitic diseases. II. Quantitative immunoenzymology].

Authors:  D Bout; J C Dugimont; H Farag; A Capron
Journal:  Lille Med       Date:  1975 Jun-Jul

4.  Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays.

Authors:  E J Ruitenberg; P A Steerenberg; B J Brosi; J Buys
Journal:  Bull World Health Organ       Date:  1974       Impact factor: 9.408

5.  Peroxidase-labeled antibody. A new method of conjugation.

Authors:  P K Nakane; A Kawaoi
Journal:  J Histochem Cytochem       Date:  1974-12       Impact factor: 2.479

6.  Immunoassay using hapten--enzyme conjugates.

Authors:  B K van Weemen; A H Schuurs
Journal:  FEBS Lett       Date:  1972-07-15       Impact factor: 4.124

7.  Determinations of proteins and hormones in serum by an immunoassay using antigen-enzyme conjugates.

Authors:  K Miedema; J Boelhouwer; J W Otten
Journal:  Clin Chim Acta       Date:  1972-08       Impact factor: 3.786

8.  Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection.

Authors:  E J Ruitenberg; I Ljungström; P A Steerenberg; J Buys
Journal:  Ann N Y Acad Sci       Date:  1975-06-30       Impact factor: 5.691

9.  Microplate enzyme-linked immunosorbent assay for chagas' disease.

Authors:  A Voller; C Draper; D E Bidwell; A Bartlett
Journal:  Lancet       Date:  1975-02-22       Impact factor: 79.321

10.  Quantitation of Salmonella O-antibodies in human sera by enzyme-linked immunosorbent assay (ELISA).

Authors:  H E Carlsson; A A Lindberg; S Hammarström; A Ljunggren
Journal:  Int Arch Allergy Appl Immunol       Date:  1975
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  38 in total

1.  Adaptation of enzyme-linked immunosorbent assay to the avian system.

Authors:  S S Slaght; T J Yang; L van der Heide
Journal:  J Clin Microbiol       Date:  1979-11       Impact factor: 5.948

2.  Evaluation of ELISA for the detection of Toxoplasma antibodies in swine sera.

Authors:  V Hirvelä-Koski
Journal:  Acta Vet Scand       Date:  1990       Impact factor: 1.695

3.  Serotyping herpes simplex virus isolated by enzyme-linked immunosorbent assays.

Authors:  K W Mills; E H Gerlach; J W Bell; M E Farkas; R J Taylor
Journal:  J Clin Microbiol       Date:  1978-01       Impact factor: 5.948

4.  Rapid indirect enzyme linked immunosorbent assay (ELISA) for detecting antitoxoplasma IgG: comparison with dye test.

Authors:  J M Francis; R A Payne; D H Joynson
Journal:  J Clin Pathol       Date:  1988-07       Impact factor: 3.411

5.  Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.

Authors:  V Pope; E F Hunter; J C Feeley
Journal:  J Clin Microbiol       Date:  1982-04       Impact factor: 5.948

6.  Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin.

Authors:  O P Surujballi; H B Fackrell
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

7.  Detection of immunoglobulin M in cerebrospinal fluid from syphilis patients by enzyme-linked immunosorbent assay.

Authors:  J B Lee; C E Farshy; E F Hunter; E A Hambie; G H Wobig; S A Larsen
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

8.  Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease.

Authors:  K Granfors
Journal:  J Clin Microbiol       Date:  1979-03       Impact factor: 5.948

9.  Enzyme-linked immunospecific antibody test for detecting antibody to Klebsiella.

Authors:  J P Rissing; T B Buxton; W L Moore; T Ozawa; W L Moore
Journal:  J Clin Microbiol       Date:  1978-12       Impact factor: 5.948

10.  Enzyme-linked immunosorbent assay for detection of pertussis immunoglobulin A in nasopharyngeal secretions as an indicator of recent infection.

Authors:  Y E Goodman; A J Wort; F L Jackson
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948
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