Literature DB >> 323223

Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.

D D Womble, D P Taylor, R H Rownd.   

Abstract

A method that gives high recovery of deoxyribonucleic acid (DNA) from crude bacterial lysates using ethidium bromide-cesium chloride density gradient centrifugation is presented. After Pronase digestion and shearing of the lysate, essentially 100% recovery of chromosomal DNA and a reproducible recovery of covalently closed circular (CCC) plasmid DNA is obtained for a specific plasmid in a given strain. This method should be useful for comparing the CCC plasmid/chromosome ratio of various plasmid-host combinations.

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Year:  1977        PMID： 323223      PMCID： PMC235186          DOI： 10.1128/jb.130.1.148-153.1977

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

1.  Properties of the relaxation complexes of supercoiled deoxyribonucleic acid and protein of the R plasmids R64, R28K, and R6K.

Authors:  Y M Kupersztoch-Portnoy; G L Miklos; D R Helinski
Journal:  J Bacteriol       Date:  1974-10       Impact factor: 3.490

2.  Genetic control of DNA initiation in Escherichia coli.

Authors:  W H Schubach; J D Whitmer; C I Davern
Journal:  J Mol Biol       Date:  1973-02-25       Impact factor: 5.469

3.  Strand-specific nick in open circular R-factor deoxyribonucleic acid: attachment of the linear strand to a proteinaceous cellular component.

Authors:  C F Morris; C L Hershberger; R Rownd
Journal:  J Bacteriol       Date:  1973-04       Impact factor: 3.490

Review 4.  Plasmid determined resistance to antibiotics: molecular properties of R factors.

Authors:  D R Helinski
Journal:  Annu Rev Microbiol       Date:  1973       Impact factor: 15.500

Review 5.  Molecular structure of bacterial plasmids.

Authors:  R C Clowes
Journal:  Bacteriol Rev       Date:  1972-09

6.  The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors:  W Bauer; J Vinograd
Journal:  J Mol Biol       Date:  1968-04-14       Impact factor: 5.469

7.  Partially supercoiled replication intermediates of R plasmid DNA are resistant to relaxation.

Authors:  D D Womble; R L Warren; R H Rownd
Journal:  Nature       Date:  1976-12-16       Impact factor: 49.962

8.  Characterization of three plasmid deoxyribonucleic acid molecules in a strain of Streptococcus faecalis: identification of a plasmid determining erythromycin resistance.

Authors:  D B Clewell; Y Yagi; G M Dunny; S K Schultz
Journal:  J Bacteriol       Date:  1974-01       Impact factor: 3.490

9.  Transduction of R factors by a Proteus mirabilis bacteriophage.

Authors:  R Nakaya; R Rownd
Journal:  J Bacteriol       Date:  1971-06       Impact factor: 3.490

10.  Round of replication mutant of a drug resistance factor.

Authors:  C F Morris; H Hashimoto; S Mickel; R Rownd
Journal:  J Bacteriol       Date:  1974-06       Impact factor: 3.490
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  50 in total

1.  Isolation and characterization of ColE1-derived plasmid copy-number mutant.

Authors:  D H Gelfand; H M Shepard; P H O'Farrell; B Polisky
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

2.  A rapid and sensitive method for plasmid copy number comparisons.

Authors:  P C Weber; M Levine; J C Glorioso
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

3.  Simple assay for quantitation of Tn5 inversion events in Escherichia coli and use of the assay in determination of plasmid copy number.

Authors:  P C Weber; M Levine; J C Glorioso
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

Review 4.  Genetic and physical map of plasmid NR1: comparison with other IncFII antibiotic resistance plasmids.

Authors:  D D Womble; R H Rownd
Journal:  Microbiol Rev       Date:  1988-12

5.  Gyrase activity and number of copies of the gyrase B subunit gene in Haemophilus influenzae.

Authors:  E Cabrera-Juárez; J K Setlow
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490

6.  A rapid and economic method for estimation of the number of plasmid copies in Escherichia coli cells.

Authors:  J Hochmannová; J Nesvera
Journal:  Folia Microbiol (Praha)       Date:  1982       Impact factor: 2.099

7.  Lincomycin stimulates synthesis of TEM-2 beta-lactamase by Escherichia coli.

Authors:  A Okabe; O Matsushita; S Katayama; H Hayashi
Journal:  Antimicrob Agents Chemother       Date:  1986-07       Impact factor: 5.191

8.  Analysis of Agrobacterium tumefaciens plasmid pTiC58 replication region with a novel high-copy-number derivative.

Authors:  D R Gallie; M Hagiya; C I Kado
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

9.  Clustering of genes involved in replication, copy number control, incompatibility, and stable maintenance of the resistance plasmid R1drd-19.

Authors:  S Molin; P Stougaard; B E Uhlin; P Gustafsson; K Nordström
Journal:  J Bacteriol       Date:  1979-04       Impact factor: 3.490

10.  Transition of deletion mutants of the composite resistance plasmid NR1 in Escherichia coli and Salmonella typhimurium.

Authors:  G A Huffman; R H Rownd
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490
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