Literature DB >> 2844732

Simple assay for quantitation of Tn5 inversion events in Escherichia coli and use of the assay in determination of plasmid copy number.

P C Weber1, M Levine, J C Glorioso.   

Abstract

In this report, we describe a simple method for measuring the frequency of sequence inversion in the transposable element Tn5 as a result of recombination across its duplicated IS50 elements. The structure of Tn5 was manipulated so that the neomycin phosphotransferase gene of the transposon would be expressed only if a sequence inversion event occurred. This highly sensitive assay also served as the basis for a novel means of estimating plasmid copy number.

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Year:  1988        PMID： 2844732      PMCID： PMC211551          DOI： 10.1128/jb.170.10.4972-4975.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Transcriptional and translational initiation sites of IS50. Control of transposase and inhibitor expression.

Authors:  M P Krebs; W S Reznikoff
Journal:  J Mol Biol       Date:  1986-12-20       Impact factor: 5.469

2.  R plasmid gene dosage effects in Escherichia coli K-12: copy mutants of the R plasmic R1drd-19.

Authors:  B E Uhlin; K Nordström
Journal:  Plasmid       Date:  1977-11       Impact factor: 3.466

3.  Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.

Authors:  D D Womble; D P Taylor; R H Rownd
Journal:  J Bacteriol       Date:  1977-04       Impact factor: 3.490

4.  Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements.

Authors:  J A Shapiro
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

5.  Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons.

Authors:  F Cabello; K Timmis; S N Cohen
Journal:  Nature       Date:  1976-01-29       Impact factor: 49.962

6.  Insertion, excision, and inversion of Tn5.

Authors:  D E Berg; C Egner; B J Hirschel; J Howard; L Johnsrud; R A Jorgensen; T D Tlsty
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1981

7.  A derivative of Tn5 with direct terminal repeats can transpose.

Authors:  B J Hirschel; D E Berg
Journal:  J Mol Biol       Date:  1982-02-25       Impact factor: 5.469

Review 8.  The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review.

Authors:  F J de Bruijn; J R Lupski
Journal:  Gene       Date:  1984-02       Impact factor: 3.688

9.  An inverted repeat sequence of the IncFI plasmid ColV2-K94 increases multimerization-mediated plasmid instability.

Authors:  P C Weber; S Palchaudhuri
Journal:  J Gen Microbiol       Date:  1986-04

10.  Yeast plasmid 2-micron circle promotes recombination within bacterial transposon Tn5.

Authors:  M Jayaram; J R Broach
Journal:  Proc Natl Acad Sci U S A       Date:  1983-12       Impact factor: 11.205
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  5 in total

1.  Analysis of Tn5 inversion events in Escherichia coli plasmids.

Authors:  P C Weber
Journal:  Mol Gen Genet       Date:  1995-08-30

2.  Replication of simian virus 40 origin-containing DNA during infection with a recombinant Autographa californica multiple nuclear polyhedrosis virus expressing large T antigen.

Authors:  D W Martin; P C Weber
Journal:  J Virol       Date:  1997-01       Impact factor: 5.103

Review 3.  Bacterial genome instability.

Authors:  Elise Darmon; David R F Leach
Journal:  Microbiol Mol Biol Rev       Date:  2014-03       Impact factor: 11.056

4.  Insertion sequence inversions mediated by ectopic recombination between terminal inverted repeats.

Authors:  Alison Ling; Richard Cordaux
Journal:  PLoS One       Date:  2010-12-20       Impact factor: 3.240

5.  Double rolling circle replication (DRCR) is recombinogenic.

Authors:  Haruko Okamoto; Taka-aki Watanabe; Takashi Horiuchi
Journal:  Genes Cells       Date:  2011-04-18       Impact factor: 1.891
  5 in total

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