Progress in Pretreatment and Analytical Methods of Coumarins: An Update since 2012 - A Review.

Coumarins are widely used due to their wide range of biological activities, but the long-term or excessive use of coumarin flavors can pose serious health hazards. Therefore, sensitive and specific methods for the quantification of these compounds in different matrices have been developed. In this review, an updated overview of the latest trends in sample preparation techniques and methods used to detect coumarins from March 2012 to April 2019 is provided. This study reviews different analytical methods (such as liquid chromatography coupled with different detectors, electrochemical sensors, capillary electrophoresis, etc.) and different pretreatment methods (such as liquid-liquid extraction, solid-phase extraction, dispersive liquid-liquid microextraction, etc.). Different methods for the pretreatment and determination of coumarins in plant, food, environmental, pharmaceutical and biological samples are summarized, discussed and compared.HighlightsProgress in pretreatment and analytical methods of coumarins are summarized.Fundamentals, instrumentation and applications of purification and quantification are summarized and compared.Optimization of experimental conditions are discussed.Newly emerged eco-friendly methods are introduced.