Literature DB >> 32314217

Identification of Long Noncoding RNA by In Situ Hybridization Approaches.

Mara Mancini1, Anna Maria Lena2, Eleonora Candi3,4.   

Abstract

In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

Entities:  

Keywords:  Fluorescence in situ hybridization; In situ hybridization; Keratinocytes; Long non coding RNA; Noncoding RNA; Skin

Mesh：

Substances：

Year:  2020        PMID： 32314217     DOI： 10.1007/978-1-0716-0648-3_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  41 in total

1.  Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing.

Authors:  D A Wassarman; J A Steitz
Journal:  Science       Date:  1992-09-25       Impact factor: 47.728

Review 2.  Annotating non-coding regions of the genome.

Authors:  Roger P Alexander; Gang Fang; Joel Rozowsky; Michael Snyder; Mark B Gerstein
Journal:  Nat Rev Genet       Date:  2010-07-13       Impact factor: 53.242

Review 3.  Non-coding RNA.

Authors:  John S Mattick; Igor V Makunin
Journal:  Hum Mol Genet       Date:  2006-04-15       Impact factor: 6.150

4.  DNA methylation silences miR-132 in prostate cancer.

Authors:  A Formosa; A M Lena; E K Markert; S Cortelli; R Miano; A Mauriello; N Croce; J Vandesompele; P Mestdagh; E Finazzi-Agrò; A J Levine; G Melino; S Bernardini; E Candi
Journal:  Oncogene       Date:  2012-02-06       Impact factor: 9.867

Review 5.  Long non-coding RNAs: insights into functions.

Authors:  Tim R Mercer; Marcel E Dinger; John S Mattick
Journal:  Nat Rev Genet       Date:  2009-03       Impact factor: 53.242

Review 6.  Function and synthesis of small nucleolar RNAs.

Authors:  D Tollervey; T Kiss
Journal:  Curr Opin Cell Biol       Date:  1997-06       Impact factor: 8.382

Review 7.  Potential of long non-coding RNAs in cancer patients: From biomarkers to therapeutic targets.

Authors:  Subash Chandra Gupta; Yashoda Nandan Tripathi
Journal:  Int J Cancer       Date:  2016-12-30       Impact factor: 7.396

Review 8.  Non-coding RNA networks in cancer.

Authors:  Eleni Anastasiadou; Leni S Jacob; Frank J Slack
Journal:  Nat Rev Cancer       Date:  2017-11-24       Impact factor: 60.716

Review 9.  Long non-coding RNAs: new players in cell differentiation and development.

Authors:  Alessandro Fatica; Irene Bozzoni
Journal:  Nat Rev Genet       Date:  2013-12-03       Impact factor: 53.242

10.  The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression.

Authors:  Thomas Derrien; Rory Johnson; Giovanni Bussotti; Andrea Tanzer; Sarah Djebali; Hagen Tilgner; Gregory Guernec; David Martin; Angelika Merkel; David G Knowles; Julien Lagarde; Lavanya Veeravalli; Xiaoan Ruan; Yijun Ruan; Timo Lassmann; Piero Carninci; James B Brown; Leonard Lipovich; Jose M Gonzalez; Mark Thomas; Carrie A Davis; Ramin Shiekhattar; Thomas R Gingeras; Tim J Hubbard; Cedric Notredame; Jennifer Harrow; Roderic Guigó
Journal:  Genome Res       Date:  2012-09       Impact factor: 9.043
View more
  1 in total

1.  Long non-coding RNA Peg13 attenuates the sevoflurane toxicity against neural stem cells by sponging microRNA-128-3p to preserve Sox13 expression.

Authors:  Yunfeng Jiang; Yue Wang; Yu Sun; Hong Jiang
Journal:  PLoS One       Date:  2020-12-09       Impact factor: 3.240
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.