Pauline Chalons1,2, Flavie Courtaut1,2, Emeric Limagne1,2,3, Fanny Chalmin1,2, Emma Cantos-Villar4, Tristan Richard5, Cyril Auger6, Philippe Chabert7, Valérie Schini-Kerth6, François Ghiringhelli1,2,3,8, Virginie Aires1,2, Dominique Delmas1,2,8. 1. Université de Bourgogne Franche-Comté, Dijon, F-21000, France. 2. INSERM Research Center U1231-Cancer and Adaptive Immune Response Team, Dijon, F-21000, France. 3. Plateforme de Transfert en Biologie du Cancer (PTBC) Centre Georges François Leclerc, Dijon, F-21000, France. 4. Instituto de Investigación y Formación Agraria y Pesquera (IFAPA) Rancho de La Merced, Ctra. Trebujena, 11.471 Jerez de la Frontera, Cadiz, Spain. 5. Université de Bordeaux, Unité de Recherche Œnologie, EA 4577, USC 1366 INRA, Equipe Molécules d'Intérêt Biologique - ISVV, Villenave-d'Ornon, F-33882, France. 6. UMR 1260 INSERM Nanomédecine Régénérative, Université de Strasbourg, Illkirch, F-67401, France. 7. UMR CNRS 7021 - Laboratoire de Bioimagerie et Pathologies, Université de Strasbourg, Illkirch-Graffenstaden, F-67401, France. 8. Department of Medical Oncology, Centre Georges François Leclerc, Dijon, F-21000, France.
Abstract
SCOPE: Scope: It is well established that immune response and inflammation promote tumoral progression. Immune cells communicate through direct contact or through cytokine secretion, and it is the pro-inflammatory status that will tip the balance toward tumor progression or anti-tumor immunity. It is demonstrated here that a red wine extract (RWE) can decrease inflammation through its action on the inflammasome complex. This study determines whether an RWE could impact other key actors of inflammation, including T helper 17 (Th17) immune cells in particular. METHODS AND RESULTS: Methods and results: Using an RWE containing 4.16 g of polyphenols/liter of wine, it is shown that RWE decreases colorectal cancer cells in vitro and induces a reduction in colorectal tumor growth associated with a decrease in tumor-infiltrating lymphocytes in vivo. The process of T-lymphocyte differentiation in Th17 cells is altered by RWE, as revealed by the decrease in the expression of key actors controlling this process, such as signal transducer and activator of transcription 3 and retinoid acid-related orphan receptor γt. This disruption is associated with an inhibition of inflammatory interleukin 17 secretion. CONCLUSION: The data highlights the major involvement of Th17 immune cells in the biological effects of an RWE.
SCOPE: Scope: It is well established that immune response and inflammation promote tumoral progression. Immune cells communicate through direct contact or through cytokine secretion, and it is the pro-inflammatory status that will tip the balance toward tumor progression or anti-tumor immunity. It is demonstrated here that a red wine extract (RWE) can decrease inflammation through its action on the inflammasome complex. This study determines whether an RWE could impact other key actors of inflammation, including T helper 17 (Th17) immune cells in particular. METHODS AND RESULTS: Methods and results: Using an RWE containing 4.16 g of polyphenols/liter of wine, it is shown that RWE decreases colorectal cancer cells in vitro and induces a reduction in colorectal tumor growth associated with a decrease in tumor-infiltrating lymphocytes in vivo. The process of T-lymphocyte differentiation in Th17 cells is altered by RWE, as revealed by the decrease in the expression of key actors controlling this process, such as signal transducer and activator of transcription 3 and retinoid acid-related orphan receptor γt. This disruption is associated with an inhibition of inflammatory interleukin 17 secretion. CONCLUSION: The data highlights the major involvement of Th17 immune cells in the biological effects of an RWE.