Literature DB >> 32304724

RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study.

G Bianco1, M Boattini2, S A V van Asten3, M Iannaccone2, E Zanotto2, T Zaccaria2, A T Bernards3, R Cavallo2, C Costa2.   

Abstract

We prospectively compared the performance of RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays directly from blood cultures spiked with 130 characterized Enterobacterales isolates. Overall, both assays yielded 100% sensitivity to detect KPC-type carbapenemases and OXA-48-like carbapenemases. Both assays failed to detect KPC-31 and KPC-33, D179Y point mutation variants of KPC-3 and KPC-2, that are deprived of carbapenemase activity and confer resistance to ceftazidime-avibactam. On blood culture bacterial pellets, NDM- and VIM-type carbapenemases were detected in 50.0% and 52.2%, respectively, by RESIST-5 O.O.K.N.V. vs 100% by NG-Test Carba 5. The sensitivity of RESIST-5 O.O.K.N.V. improved to 100% and 95.6%, respectively, by performing the assay on 4-h early subculture.
Copyright © 2020. Published by Elsevier Ltd.

Entities:  

Keywords:  Blood culture; Carbapenemase detection; Ceftazidime-avibactam resistance; D179Y; Enterobacterales; Immunochromatographic assay

Mesh：

Substances：

Year:  2020        PMID： 32304724     DOI： 10.1016/j.jhin.2020.03.022

Source DB:  PubMed          Journal:  J Hosp Infect        ISSN： 0195-6701            Impact factor:   3.926


  9 in total

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Authors:  Matteo Boattini; Gabriele Bianco; Marco Iannaccone; Davide Ghibaudo; André Almeida; Rossana Cavallo; Cristina Costa
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5.  Evaluation of the Immunochromatographic NG-Test Carba 5, RESIST-5 O.O.K.N.V., and IMP K-SeT for Rapid Detection of KPC-, NDM-, IMP-, VIM-type, and OXA-48-like Carbapenemase Among Enterobacterales.

Authors:  Renru Han; Yan Guo; Mingjia Peng; Qingyu Shi; Shi Wu; Yang Yang; Yonggui Zheng; Dandan Yin; Fupin Hu
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6.  Direct detection of extended-spectrum-β-lactamase-producers in Enterobacterales from blood cultures: a comparative analysis.

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9.  Occurrence of multi-carbapenemases producers among carbapenemase-producing Enterobacterales and in vitro activity of combinations including cefiderocol, ceftazidime-avibactam, meropenem-vaborbactam, and aztreonam in the COVID-19 era.

Authors:  Gabriele Bianco; Matteo Boattini; Sara Comini; Roberto Casale; Marco Iannaccone; Rossana Cavallo; Cristina Costa
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  9 in total

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