Theresa W Chan1, Simone Langness1, Olga Cohen1, Brian P Eliceiri1, Andrew Baird1, Todd W Costantini2. 1. Division of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA. 2. Division of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, University of California San Diego School of Medicine, 200 W. Arbor Drive #8896, San Diego, CA, 92103, USA. tcostantini@health.ucsd.edu.
Abstract
OBJECTIVE AND DESIGN: CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration. MATERIAL: Human THP-1, RAW264.7 and HEK293 cells. METHODS: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. RESULTS: We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. CONCLUSION: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.
OBJECTIVE AND DESIGN:CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration. MATERIAL: HumanTHP-1, RAW264.7 and HEK293 cells. METHODS: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. RESULTS: We show that gene delivery of CHRFAM7A into the THP-1human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. CONCLUSION: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.
Authors: Todd W Costantini; Xitong Dang; Maryana V Yurchyshyna; Raul Coimbra; Brian P Eliceiri; Andrew Baird Journal: Mol Med Date: 2015-04-03 Impact factor: 6.354
Authors: Todd W Costantini; Theresa W Chan; Olga Cohen; Simone Langness; Sabrina Treadwell; Elliot Williams; Brian P Eliceiri; Andrew Baird Journal: Proc Natl Acad Sci U S A Date: 2019-04-03 Impact factor: 11.205