Literature DB >> 3230141

Early detection of influenza virus by using a fluorometric assay of infected tissue culture.

C T Pachucki1, C Creticos.   

Abstract

A fluorometric substrate, 4-methylumbelliferyl-alpha-ketoside of N-acetylneuramide, was used directly on clinical specimens and infected tissue culture 24 h after inoculation for the detection of influenza viral neuraminidase. Viral neuraminidase was detected in infected tissue culture but not in clinical specimens. The sensitivity of the assay on tissue culture was 92%, and the specificity was 96%.

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Year:  1988        PMID： 3230141      PMCID： PMC266969          DOI： 10.1128/jcm.26.12.2664-2666.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  The synthesis of 4-methylumbelliferyl alpha-ketoside of N-acetylneuraminic acid and its use in a fluorometric assay for neuraminidase.

Authors:  R W Myers; R T Lee; Y C Lee; G H Thomas; L W Reynolds; Y Uchida
Journal:  Anal Biochem       Date:  1980-01-01       Impact factor: 3.365

2.  Early testing of cell cultures for detection of hemadsorbing viruses.

Authors:  L L Minnich; C G Ray
Journal:  J Clin Microbiol       Date:  1987-02       Impact factor: 5.948

3.  Time-resolved fluoroimmunoassay with monoclonal antibodies for rapid diagnosis of influenza infections.

Authors:  H H Walls; K H Johansson; M W Harmon; P E Halonen; A P Kendal
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

4.  Fluorometric assay for measurement of viral neuraminidase--application to the rapid detection of influenza virus in nasal wash specimens.

Authors:  R H Yolken; V M Torsch; R Berg; B R Murphy; Y C Lee
Journal:  J Infect Dis       Date:  1980-10       Impact factor: 5.226

5.  Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B viruses.

Authors:  H H Walls; M W Harmon; J J Slagle; C Stocksdale; A P Kendal
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948
  5 in total
  2 in total

1.  Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.

Authors:  M Seno; Y Kanamoto; S Takao; N Takei; S Fukuda; H Umisa
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

2.  Integrated Microfluidic Preconcentration and Nucleic Amplification System for Detection of Influenza A Virus H1N1 in Saliva.

Authors:  Yonghee Kim; Abdurhaman Teyib Abafogi; Buu Minh Tran; Jaewon Kim; Jinyeop Lee; Zhenzhong Chen; Pan Kee Bae; Kyoungsook Park; Yong-Beom Shin; Danny van Noort; Nae Yoon Lee; Sungsu Park
Journal:  Micromachines (Basel)       Date:  2020-02-16       Impact factor: 2.891
  2 in total

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