Literature DB >> 3230134

Calculation of antibody affinity in homogeneous and heterogeneous systems.

R R Chalquest1.   

Abstract

Antibody affinity is an important determinant of all antibody-antigen reactions. A new computer program, AFCRV, was developed to calculate binding constants with data from a radioimmunoassay on most microcomputers in the laboratory by using constant-ratio dilution curves. Evaluation of a homogeneous or heterogeneous antibody in the presence of a single antigen can be accomplished.

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Year:  1988        PMID： 3230134      PMCID： PMC266946          DOI： 10.1128/jcm.26.12.2561-2563.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  The importance of antibody affinity in the performance of immunoassays for antibody.

Authors:  M W Steward; A M Lew
Journal:  J Immunol Methods       Date:  1985-04-22       Impact factor: 2.303

2.  Computer modeling of the reaction between antigen and heterogeneous antibody in fixed antigen excess: a new method for measuring antibody affinity.

Authors:  D P Nelson; W R Griswold
Journal:  J Clin Lab Immunol       Date:  1986-10

3.  A simple method for ranking the affinities of monoclonal antibodies.

Authors:  V Van Heyningen; D J Brock; S Van Heyningen
Journal:  J Immunol Methods       Date:  1983-08-26       Impact factor: 2.303

4.  Quantitative explanation for increased affinity shown by mixtures of monoclonal antibodies: importance of a circular complex.

Authors:  W R Moyle; C Lin; R L Corson; P H Ehrlich
Journal:  Mol Immunol       Date:  1983-04       Impact factor: 4.407

5.  Numbers of receptor sites from Scatchard graphs: facts and fantasies.

Authors:  I M Klotz
Journal:  Science       Date:  1982-09-24       Impact factor: 47.728

6.  Analyzing nonlinear scatchard plots.

Authors:  K E Light
Journal:  Science       Date:  1984-01-06       Impact factor: 47.728

7.  Influence of antibody affinity on the performance of different antibody assays.

Authors:  G R Nimmo; A M Lew; C M Stanley; M W Steward
Journal:  J Immunol Methods       Date:  1984-08-03       Impact factor: 2.303

8.  Characterization of monoclonal antibodies against human chorionic somatomammotropin: competitive screening and determination of the affinity constants.

Authors:  M Mariani; P Neri; M Cianfriglia; D Armellini; G Barbarulli; G Antoni
Journal:  J Immunol Methods       Date:  1984-06-08       Impact factor: 2.303

9.  Affinity requirements for antibody assays mapped by monoclonal antibodies.

Authors:  F Péterfy; P Kuusela; O Mäkelä
Journal:  J Immunol       Date:  1983-04       Impact factor: 5.422

10.  Studies on B lymphoid tumors treated with monoclonal anti-idiotype antibodies: correlation with clinical responses.

Authors:  J N Lowder; T C Meeker; M Campbell; C F Garcia; J Gralow; R A Miller; R Warnke; R Levy
Journal:  Blood       Date:  1987-01       Impact factor: 22.113
  10 in total
  1 in total

1.  Establishment of reverse direct ELISA and its application in screening high-affinity monoclonal antibodies.

Authors:  Chunmei Zhang; Rende Li; Yongming Li; Chaojun Song; Zhijia Liu; Yun Zhang; Zhuwei Xu; Ran Zhuang; Jing Yi; Angang Yang; Kun Yang; Boquan Jin
Journal:  Hybridoma (Larchmt)       Date:  2012-08
  1 in total

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