Lei Yang1, Flavia Teles2, Weida Gong3, Shawn A Dua4, Lynn Martin2, Mark H Schoenfisch5. 1. Department of Chemistry, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, United States. 2. School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States. 3. Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, United States. 4. School of Dentistry, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, United States. 5. Department of Chemistry, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, United States. Electronic address: schoenfisch@unc.edu.
Abstract
OBJECTIVES: This study investigates the antibiofilm action of nitric oxide (NO)-releasing hyperbranched polymers against ex vivo multispecies periodontal biofilms. METHODS: The antibiofilm efficacy of NO-releasing hyperbranched polymers was evaluated as a function of NO-release properties, polymer concentrations, and oxygen levels in the exposure media. 16s rRNA sequencing technique was employed to evaluate the impact of NO-releasing hyperbranched polymers on the microbial composition of the biofilms. RESULTS: The addition of NO release significantly improved the antibiofilm action of the hyperbranched polymers, with NO-releasing hyperbranched polyamidoamines of largest NO payloads being more effective than hyperbranched polykanamycins. Furthermore, the NO-releasing hyperbranched polymers reduced the biofilm metabolic activity in a dose-dependent manner, killing biofilm-detached bacteria under both aerobic and anaerobic conditions, with greater antimicrobial efficacy observed under aerobic conditions. SIGNIFICANCE: These results demonstrate for the first time the potential therapeutic utility of NO-releasing hyperbranched polymers for treating multispecies dental biofilms.
OBJECTIVES: This study investigates the antibiofilm action of nitric oxide (NO)-releasing hyperbranched polymers against ex vivo multispecies periodontal biofilms. METHODS: The antibiofilm efficacy of NO-releasing hyperbranched polymers was evaluated as a function of NO-release properties, polymer concentrations, and oxygen levels in the exposure media. 16s rRNA sequencing technique was employed to evaluate the impact of NO-releasing hyperbranched polymers on the microbial composition of the biofilms. RESULTS: The addition of NO release significantly improved the antibiofilm action of the hyperbranched polymers, with NO-releasing hyperbranched polyamidoamines of largest NO payloads being more effective than hyperbranched polykanamycins. Furthermore, the NO-releasing hyperbranched polymers reduced the biofilm metabolic activity in a dose-dependent manner, killing biofilm-detached bacteria under both aerobic and anaerobic conditions, with greater antimicrobial efficacy observed under aerobic conditions. SIGNIFICANCE: These results demonstrate for the first time the potential therapeutic utility of NO-releasing hyperbranched polymers for treating multispecies dental biofilms.
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