Stefan Maenz1, Olaf Brinkmann2,3, Raimund W Kinne4, Matthias Bungartz2,3, Ines Hasenbein3, Christina Braun3, Elke Kunisch3, Victoria Horbert3, Francesca Gunnella3, André Sachse3, Sabine Bischoff5, Harald Schubert5, Klaus D Jandt1, Jörg Bossert1, Dominik Driesch6. 1. Chair of Materials Science, Otto Schott Institute of Materials Research, Friedrich-Schiller-University Jena, Jena, Germany. 2. Chair of Orthopedics, Department of Orthopedics, Jena University Hospital, Waldkliniken Eisenberg GmbH, Eisenberg, Germany. 3. Experimental Rheumatology Unit, Department of Orthopedics, Jena University Hospital, Waldkliniken Eisenberg GmbH, Klosterlausnitzer Str. 81, 07607, Eisenberg, Germany. 4. Experimental Rheumatology Unit, Department of Orthopedics, Jena University Hospital, Waldkliniken Eisenberg GmbH, Klosterlausnitzer Str. 81, 07607, Eisenberg, Germany. raimund.w.kinne@med.uni-jena.de. 5. Institute of Laboratory Animal Sciences and Welfare, Jena University Hospital, Jena, Germany. 6. BioControl Jena GmbH, Jena, Germany.
Abstract
INTRODUCTION: Existing osteoporosis models in sheep exhibit some disadvantages, e.g., challenging surgical procedures, serious ethical concerns, failure of reliable induction of substantial bone loss, or lack of comparability to the human condition. This study aimed to compare bone morphological and mechanical properties of old and young sheep, and to evaluate the suitability of the old sheep as a model for senile osteopenia. MATERIALS AND METHODS: The lumbar vertebral body L3 of female merino sheep with two age ranges, i.e., old animals (6-10 years; n = 41) and young animals (2-4 years; n = 40), was analyzed concerning its morphological and mechanical properties by bone densitometry, quantitative histomorphometry, and biomechanical testing of the corticalis and/or central spongious region. RESULTS: In comparison with young sheep, old animals showed only marginally diminished bone mineral density of the vertebral bodies, but significantly decreased structural (bone volume, - 15.1%; ventral cortical thickness, - 11.8%; lateral cortical thickness, - 12.2%) and bone formation parameters (osteoid volume, osteoid surface, osteoid thickness, osteoblast surface, all - 100.0%), as well as significantly increased bone erosion (eroded surface, osteoclast surface). This resulted in numerically decreased biomechanical properties (compressive strength; - 6.4%). CONCLUSION: Old sheep may represent a suitable model of senile osteopenia with markedly diminished bone structure and formation, and substantially augmented bone erosion. The underlying physiological aging concept reduces challenging surgical procedures and ethical concerns and, due to complex alteration of different facets of bone turnover, may be well representative of the human condition.
INTRODUCTION: Existing osteoporosis models in sheep exhibit some disadvantages, e.g., challenging surgical procedures, serious ethical concerns, failure of reliable induction of substantial bone loss, or lack of comparability to the human condition. This study aimed to compare bone morphological and mechanical properties of old and young sheep, and to evaluate the suitability of the old sheep as a model for senile osteopenia. MATERIALS AND METHODS: The lumbar vertebral body L3 of female merino sheep with two age ranges, i.e., old animals (6-10 years; n = 41) and young animals (2-4 years; n = 40), was analyzed concerning its morphological and mechanical properties by bone densitometry, quantitative histomorphometry, and biomechanical testing of the corticalis and/or central spongious region. RESULTS: In comparison with young sheep, old animals showed only marginally diminished bone mineral density of the vertebral bodies, but significantly decreased structural (bone volume, - 15.1%; ventral cortical thickness, - 11.8%; lateral cortical thickness, - 12.2%) and bone formation parameters (osteoid volume, osteoid surface, osteoid thickness, osteoblast surface, all - 100.0%), as well as significantly increased bone erosion (eroded surface, osteoclast surface). This resulted in numerically decreased biomechanical properties (compressive strength; - 6.4%). CONCLUSION: Old sheep may represent a suitable model of senile osteopenia with markedly diminished bone structure and formation, and substantially augmented bone erosion. The underlying physiological aging concept reduces challenging surgical procedures and ethical concerns and, due to complex alteration of different facets of bone turnover, may be well representative of the human condition.
Entities:
Keywords:
Large animal model; Old sheep; Senile osteopenia; Senile osteoporosis
Authors: A Sachse; A Wagner; M Keller; O Wagner; W-D Wetzel; F Layher; R-A Venbrocks; P Hortschansky; M Pietraszczyk; B Wiederanders; H J Hempel; J Bossert; J Horn; K Schmuck; J Mollenhauer Journal: Bone Date: 2005-09-01 Impact factor: 4.398
Authors: Matthias Bungartz; Stefan Maenz; Elke Kunisch; Victoria Horbert; Long Xin; Francesca Gunnella; Joerg Mika; Juliane Borowski; Sabine Bischoff; Harald Schubert; Andre Sachse; Bernhard Illerhaus; Jens Günster; Jörg Bossert; Klaus D Jandt; Raimund W Kinne; Olaf Brinkmann Journal: Spine J Date: 2016-06-23 Impact factor: 4.166
Authors: Ines Hasenbein; André Sachse; Peter Hortschansky; Klaus D Schmuck; Victoria Horbert; Christoph Anders; Thomas Lehmann; René Huber; Alexander Maslaris; Frank Layher; Christina Braun; Andreas Roth; Frank Plöger; Raimund W Kinne Journal: Biomedicines Date: 2022-02-21