Thuỳ Linh Phạm1,2, Yuhua Yin1,2,3, Hyeok Hee Kwon1,2, Nara Shin1,2, Song I Kim1,2, Hyewon Park1,2, Juhee Shin1,2, Hyo Jung Shin1,2, Jeong-Ah Hwang1,2, Hee-Jung Song4, Sang Ryong Kim5, Joo Hyoung Lee6, Patrick T J Hwang7, Ho-Wook Jun7, Dong Woon Kim1,2. 1. Department of Medical Science, Chungnam National University College of Medicine, Daejeon, 35015, Republic of Korea. 2. Department of Anatomy and Cell Biology, Brain Research Institute, Chungnam National University College of Medicine, Daejeon, 35015, Republic of Korea. 3. Department of Anesthesia, Guangzhou Women and Children's Medical Center, Guangzhou, 510623, Guangdong Province, PR China. 4. Department of Neurology, Brain Research Institute, Chungnam National University College of Medicine, Daejeon, 35015, Republic of Korea. 5. School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Institute of Life Science & Biotechnology, Brain Science & Engineering Institute, Kyungpook National University, Daegu, 41566, Republic of Korea. 6. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. 7. Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Abstract
Aims: We investigated whether miRNA (miR) 146a-5p-loaded nanoparticles (NPs) can attenuate neuropathic pain behaviors in the rat spinal nerve ligation-induced neuropathic pain model by inhibiting activation of the NF-κB and p38 MAPK pathways in spinal microglia. Materials & methods: After NP preparation, miR NPs were assessed for their physical characteristics and then injected intrathecally into the spinal cords of rat spinal nerve ligation rats to test their analgesic effects. Results: miR NPs reduced pain behaviors for 11 days by negatively regulating the inflammatory response in spinal microglia. Conclusion: The anti-inflammatory effects of miR 146a-5p along with nanoparticle-based materials make miR NPs promising tools for treating neuropathic pain.
Aims: We investigated whether miRNA (miR) 146a-5p-loaded nanoparticles (NPs) can attenuate neuropathic pain behaviors in the rat spinal nerve ligation-induced neuropathic pain model by inhibiting activation of the NF-κB and p38 MAPK pathways in spinal microglia. Materials & methods: After NP preparation, miR NPs were assessed for their physical characteristics and then injected intrathecally into the spinal cords of rat spinal nerve ligation rats to test their analgesic effects. Results: miR NPs reduced pain behaviors for 11 days by negatively regulating the inflammatory response in spinal microglia. Conclusion: The anti-inflammatory effects of miR 146a-5p along with nanoparticle-based materials make miR NPs promising tools for treating neuropathic pain.