A family cluster of severe acute respiratory syndrome coronavirus 2 infections.

Clinical and epidemiological knowledge of 2019 novel coronavirus disease (COVID-19) is limited. We reported a family cluster of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases in Beijing, China. This family comprised three laboratory confirmed cases with clinical symptoms. All three patients had close contact with a relative from Wuhan, Hubei Province. Throat swab samples were all positive for SARS-CoV-2 using real-time reverse transcriptase-polymerase chain reaction assays. Chest computerized tomography revealed ground-glass opacities and consolidation. SARS-CoV-2 infections tend to clusters. Physicians should be aware of contact history so that infected patients can be identified promptly and further spreading prevented.

In December 19, a pneumonia called 2019 novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, Hubei Province, China [1, 2]. As of February 20, 2020, there have been a total of 74,675 confirmed cases in China and 1073 confirmed cases outside of China [3]. Although SARS-CoV-2 was thought to have a zoonotic origin and early cases were reported to be associated with exposure to the seafood market in Wuhan, person-to-person transmission has been observed [4, 5]. At present, the emergence, development, and underlying mechanism of immunity and clinical outcomes of patients with COVID-19 outside Wuhan are limited. Here, we report the epidemiologic and clinical features of a family cluster of SARS-CoV-2 infections that occurred in Beijing who did not have a history of travel to Wuhan.

Patient 1 was a 72-year-old female with hypertension. She presented to the emergency department with 10 h of fever (maximum temperature of 38.1 °C) and muscle soreness in the late evening of February 2, 2020. The patient’s oxygen saturation was 98% while she was breathing ambient air. A chest CT scan showed ground-glass opacities in the upper lobe of the right lung (Fig. 1). After admission, she initially received antiviral therapy with lopinavir/ritonavir and aerosol inhalation of interferon-alpha in addition to supplemental oxygen delivered by nasal cannula at 2 L per min. She was diagnosed with COVID-19 because her throat swab sample was nucleic acid–positive for SARS-CoV-2 [6]. Laboratory results are summarized in Table 1. On the second hospital day, she developed intermittent atrial fibrillation. She was treated with low dose of betaloc. The patient was still feverish with the maximum temperature of 39 °C, and she received antipyretic therapy. A second chest CT revealed bilateral lung disease with an increasing number of ground-glass opacities and a crazy-paving pattern in February 7. In February 10, she complained of worsening dyspnea. Oxygen saturation decreased to 90–92% while the patient was breathing 10 L of oxygen with the use of an oxygen mask. Blood-gas evaluations showed type I respiratory failure. She was transferred to the intensive care unit (ICU) immediately, where she received high-flow nasal oxygen therapy. She was finally intubated and given mechanical ventilation for severe acute respiratory distress syndrome (ARDS) in February 14. A third chest CT showed decreased ground-glass opacities, and her fever finally resolved in February 19. However, her throat swab sample was still nucleic acid–positive in February 19.

Fig. 1

Chest CT of patient 1

Table 1

Clinical laboratory results of patients with COVID-19 on admission to hospital

Variables	Case 1	Case 2	Case 3	Reference range
Age	72	47	45
Gender	Female	Male	Female
White blood cell count (× 109/L)	2.99	2.96	4.9	4–10
Neutrophil count (× 109/L)	1.98	1.63	2.94	2–8
Lymphocyte count (× 109/L)	0.76	1.07	1.63	1–5
Eosinophil count (× 109/L)	0.01	0.00	0.01	0.02–0.5
Hemoglobin (g/L)	131	157	109	110–150
Platelet count (× 109/L)	156	133	263.4	100–300
Alanine aminotransferase (U/L)	18.9	83.7	25.1	7–40
Alanine aminotransferase (U/L)	19.5	46.3	28.1	13–35
Total bilirubin (μmol/L)	9.7	8.3	5.5	0–18.8
Creatine (μmol/L)	61.1	88.1	51.9	Male, 57–97 female, 41–73
C-Reactive protein (mg/L)	2.7	7.4	1.4	0–5
Lactate dehydrogenase (U/L)	161.4	200.9	366.9	120–250
Creatine kinase (U/L)	38.1	72.2	111.1	40–200
Creatine kinase-MB (U/L)	16.3	14.2	50.1	< 25
α-Hydroxybutyric dehydrogenase (U/L)	130	155	324	74–182
Troponin I (μg/L)	< 0.01	< 0.01	< 0.01	< 0.01
Procalcitonin (ng/mL)	< 0.05	< 0.05	< 0.05	< 0.05
Serum amyloid A (mg/L)	6.3	106.4	6.5	0–10
Prothrombin time (s)	12.4	13.3	12	9.4–12.5
International normalized ratio	1.15	1.23	1.11	0.8–1.2
d-Dimer (mg/L)	0.76	0.31	0.50
CD4 (cell/μL)	180	285	511	706–1125
CD8 (cell/μL)	253	126	729	320–1250
NK (cell/μL)	37	51	444	90–590
B (cell/μL)	147	104	189	90–660
Lactate (mmol/L)	2.05	2.05	1.48	1.33–1.78

Patient 2 was a 47-year-old male and the son of patient 1. They presented to the emergency department on February 2, 2020. He complained of a 5-day history of high fever (maximum temperature of 39.2 °C), and cough. A chest CT scan showed bilateral ground-glass opacities with consolidation (Fig. 2). A throat swab nucleic acid test was positive for SARS-CoV-2. After admission, he was also treated with lopinavir/ritonavir and aerosol inhalation of interferon-alpha. However, his condition deteriorated with high fever (40.0 °C) and shortness of breath. A second chest CT showed extensive bilateral ground-glass opacities and consolidation in February 8. Blood-gas evaluations showed type I respiratory failure. Then, he was transferred to the ICU with ARDS. The patient’s oxygenation improved with high-flow nasal oxygen. His fever resolved in February 9. His nucleic acid test became negative after February 15. His clinical conditions improved, and he was transferred back to the general ward in February 18. He continued to receive supplemental oxygen delivered by nasal cannula at 2 L per min. His oxygen saturation remained at 98%.

Fig. 2

Chest CT of patient 2

Patient 3 was a 45-year-old female and the wife of patient 2. They presented to the emergency department on February 2, 2020. She complained of having fever (maximum temperature of 37.7 °C) and cough for 5 days. A chest CT scan showed bilateral ground-glass opacities and consolidation in the lower right lobe (Fig. 3). A throat swab sample was positive for SARS-CoV-2. She received lopinavir/ritonavir and aerosol inhalation of interferon-alpha. Her fever resolved from the second hospital day. Results of routine blood counts and blood-gas evaluations were normal. A chest CT scan revealed partial absorption of the lesion in February 14. However, she was still hospitalized due to the persistent positive nucleic acid tests.

Fig. 3

Chest CT of patient 3

Here we report a familial cluster of pneumonia due to SARS-CoV-2 infection. The family cluster of 3 patients (patients 1–3) drove from Beijing to Xinyang, Henan Province, on January 24, 2020. The family stayed in their own house throughout their trip. They shared meals with a relative from Wuhan on January 27, 2020. They had no history of contact with other people with fever or respiratory tract symptoms, contact with wild animals, visits to the Huanan seafood market in Wuhan, or consumption of game meat in restaurants. After they drove back to Beijing on January 27, 2020, the 3 patients lived in one apartment. Patient 1 stayed in one room while patients 2 and 3 stayed in another. Patients 2–3 presented with fever and cough on January 29, 2020. Patient 1 presented with fever on February 2, 2020. The incubation period was estimated to be between 2 and 6 days. The most likely course of transmission was that patients 1–3 acquired the infection from their relative from Wuhan, while sharing meals. Their relative might has acquired the infection from the community, as he had no clear exposure history. According to what they had been told, the relative from Wuhan was isolated. However, he did not receive a nucleic acid test because he has no symptoms.

All three patients successively presented with fever. The symptoms of this COVID-19 are also nonspecific, and fever is the most common symptom [7, 8]. Although patients 1–2 had mild symptoms at the time of admission, their dyspnea worsened in 7–10 days from the time of onset. The dynamic changes in chest CT scans were also consistent with clinical course. Therefore, radiological assessment of patients’ progress plays an important role in the management of COVID-19. Currently, no specific antiviral treatment exists. Clinical management focuses on symptomatic treatment, with organ support for critically ill patients. Lopinavir/ritonavir and aerosol inhalation of interferon-alpha were used as soon as they were admitted. However, their clinical courses diverged. The conditions of patient 1 became aggravated with positive nucleic acid tests; however, patient 3 resolved with a positive nucleic acid test. Further randomized controlled trials are needed to evaluate efficacy of these antiviral medications, including remdesivir [9]. The pathophysiology mechanism of severity levels caused by SARS-CoV-2 is not well understood. It has been found to be associated with a cytokine storm, which is characterized by increased plasma concentrations of IL-2, IL-7, IL-10, MCP-1, MIP-1α, and TNF-α in critical patients according to previous studies [10-12]. Xu et al. postulated overactivation of T cells may partly account for the severe immune injury [13]. These excessive immune responses lead to further lung injury. Corticosteroids were used frequently to treat severe patients. Huang et al. treated few severely ill patients with ARDS with low-to-moderate doses of corticosteroids [11]. Systemic corticosteroid was given to 44.5% of severe cases and 13.7% of non-severe cases [7]. However, none of these treatments were found to be effective [8, 14]. In this setting, administration of corticosteroids has been conditionally recommended in our guidance [6], but it has not been recommended by the WHO [15]. Intravenous injection with γ-immunoglobulin may be an alternative [14]. However, more clinical data are required to evaluate it.

In conclusion, cases of COVID-19 tend to emerge in clusters. No specific therapeutics are available for treatment of COVID-19. Physicians should be aware of contact history for patients to promptly identify patients and prevent further spreading. Early diagnosis, early isolation, and early management are crucial to curbing transmission and reducing mortality.