M Muñoz1, J Gatien2, P Salvetti2, D Martín-González3, S Carrocera3, E Gómez3. 1. Centro de Biotecnología Animal - SERIDA, Camino de Rioseco, 1225, Gijón, Spain. mmunoz@serida.org. 2. ALLICE, Experimental Facilities, Le Perroi, 37380, Nouzilly, France. 3. Centro de Biotecnología Animal - SERIDA, Camino de Rioseco, 1225, Gijón, Spain.
Abstract
INTRODUCTION: Bovine female and male embryos differentially release metabolites with signalling effects to culture media. However, it is unknown if the embryo-maternal interface (EMI) metabolome is modified by embryonic sex. OBJECTIVE: To analyse using a combination of 1H NMR and a co-culture of endometrial cells the EMI. RESULTS: Twenty-six metabolites were identified and quantified in the EMI, nine metabolites reflected the sex of the embryo rather than their presence. CONCLUSIONS: 1H NMR is sensitive enough to perform quantitative analysis of sex-induced differences in the EMI. These results may help to understand the embryo-maternal dialogue on the basis of embryonic sex.
INTRODUCTION:Bovine female and male embryos differentially release metabolites with signalling effects to culture media. However, it is unknown if the embryo-maternal interface (EMI) metabolome is modified by embryonic sex. OBJECTIVE: To analyse using a combination of 1H NMR and a co-culture of endometrial cells the EMI. RESULTS: Twenty-six metabolites were identified and quantified in the EMI, nine metabolites reflected the sex of the embryo rather than their presence. CONCLUSIONS:1H NMR is sensitive enough to perform quantitative analysis of sex-induced differences in the EMI. These results may help to understand the embryo-maternal dialogue on the basis of embryonic sex.
Entities:
Keywords:
1H NMR; Bovine; Embryo; Endometrial cells; Sex
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