Yunsheng Dong1, Huahong Zhuang1, Yan Hao1, Lin Zhang1, Qiang Yang2, Yufei Liu1, Chunxiao Qi1, Shufang Wang1. 1. State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, The College of Life Science, Nankai University, Tianjin, People's Republic of China. 2. Department of Spine Surgery, Tianjin Hospital, Tianjin, People's Republic of China.
Abstract
INTRODUCTION: Chronic trauma repair is an important issue affecting people's healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair. METHODS: The purpose of this research was to prepare a novel thermo-sensitive hydrogel-poly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining. RESULTS: Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model. DISCUSSION: The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.
INTRODUCTION: Chronic trauma repair is an important issue affecting people's healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair. METHODS: The purpose of this research was to prepare a novel thermo-sensitive hydrogel-poly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining. RESULTS: Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model. DISCUSSION: The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.
Authors: Settimio Pacelli; Francisca Acosta; Aparna R Chakravarti; Saheli G Samanta; Jonathan Whitlow; Saman Modaresi; Rafeeq P H Ahmed; Johnson Rajasingh; Arghya Paul Journal: Acta Biomater Date: 2017-05-19 Impact factor: 8.947
Authors: Cristina Gentilini; Yixiang Dong; Jessica R May; Silvia Goldoni; David E Clarke; Boon-Heng Lee; E Thomas Pashuck; Molly M Stevens Journal: Adv Healthc Mater Date: 2012-04-05 Impact factor: 9.933
Authors: Viviana R Güiza-Argüello; Víctor A Solarte-David; Angie V Pinzón-Mora; Jhair E Ávila-Quiroga; Silvia M Becerra-Bayona Journal: Polymers (Basel) Date: 2022-07-06 Impact factor: 4.967