Ana L Riveros1,2, Cynthia Eggeling3, Sebastián Riquelme1, Carolina Adura1, Carmen López-Iglesias4, Fanny Guzmán3, Eyleen Araya5, Mario Almada6, Josué Juárez6, Miguel A Valdez6, Ignacio A Fuentevilla1,2,7, Olga López8, Marcelo J Kogan1,2. 1. Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago, Chile. 2. Advanced Center for Chronic Diseases (ACCDiS), Universidad de Chile, Santiago, Chile. 3. Núcleo de Biotecnología Curauma (NBC), Universidad Católica de Valparaíso, Valparaíso, Chile. 4. Microscopy CORE Lab, The Maastricht Multimodal Molecular Imaging Institute FHML, Maastricht University, Maastrich, Netherlands. 5. Departamento de Ciencias Quimicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Santiago, Chile. 6. Departamento de Física, Universidad de Sonora, Hermosillo, Sonora, México. 7. Laboratorio de Investigación en nutrición funcional (LINF), Instituto de Nutrición y Tecnología de los alimentos (INTA), Universidad de Chile, Santiago, Chile. 8. Department Surfactants and Nanobiotechnology, Institute for advanced chemistry of Catalonia, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), Barcelona, Spain.
Abstract
INTRODUCTION: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD. METHODS: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM. RESULTS AND DISCUSSION: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage. CONCLUSION: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.
INTRODUCTION: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD. METHODS: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM. RESULTS AND DISCUSSION: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage. CONCLUSION: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.
Authors: Carolina Adura; Simon Guerrero; Edison Salas; Luis Medel; Ana Riveros; Juan Mena; Jordi Arbiol; Fernando Albericio; Ernest Giralt; Marcelo J Kogan Journal: ACS Appl Mater Interfaces Date: 2013-05-06 Impact factor: 9.229
Authors: Biao Chen; Xiaoqi Dong; Xiyuan Dong; Quan Wang; Meng Wu; Jun Wu; Xiaoding Lou; Fan Xia; Wenwen Wang; Jun Dai; Shixuan Wang Journal: Int J Nanomedicine Date: 2021-02-18