| Literature DB >> 32255848 |
Sanath Kondaveeti1, Sanjay K S Patel1, Janghun Woo1, Ji Hyang Wee2, Sang-Yong Kim2, Riyadh I Al-Raoush3, In-Won Kim1, Vipin Chandra Kalia1, Jung-Kul Lee1.
Abstract
A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (V max = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity. © Association of Microbiologists of India 2019.Entities:
Keywords: Catalytic efficiency; Cellobiohydrolase; Enzyme production; Enzyme purification; Schizophyllum commune
Year: 2019 PMID： 32255848 PMCID： PMC7105533 DOI： 10.1007/s12088-019-00843-9
Source DB: PubMed Journal: Indian J Microbiol ISSN： 0046-8991 Impact factor: 2.461