Zi-Jian Chen1, Xi-Xia Liu2, Zhi-Li Xiao3, Hui-Jun Fu4, Yu-Ping Huang5, Shu-Yi Huang6, Yu-Dong Shen7, Fan He8, Xing-Xing Yang9, Bruce Hammock10, Zhen-Lin Xu11. 1. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: czj1q2w3e4r5t@163.com. 2. Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Hubei Normal University, Huangshi, 435002, China. Electronic address: liuxixia1@163.com. 3. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: scau_xzl@163.com. 4. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: Huijun.FOOO@gmail.com. 5. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: HHYP823512841@163.com. 6. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: huangshuyi9246@163.com. 7. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: shenyudong@scau.edu.cn. 8. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: snowywhite@163.com. 9. Shenzhen Bioeasy Biotechnology Co., Ltd., Shenzhen, 518102, China. Electronic address: xyysz1111@163.com. 10. Department of Entomology and UCD Comprehensive Cancer Center, University of California, Davis, CA, 95616, United States. Electronic address: bdhammock@ucdavis.edu. 11. Guangdong Provincial Key Laboratory of Food (1)uality and Safety, South China Agricultural University, Guangzhou, 510642, China. Electronic address: jallent@163.com.
Abstract
1-naphthol (1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organic compound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.
1-naphthol (n class="Chemical">1-NAP) is the main metabolite of pesticide carbaryl and naphthalene, and is also a genotoxic and carcinogenic intermediate in the synthesis of organiccompound, dyes, pigment and pharmaceutical industry. In this work, two novel haptens were designed and synthesized for developing a competitive indirect enzyme-linked immunosorbent assay (ciELISA) method for 1-NAP in urine samples. The assay showed a limit of detection of 2.21 ng/mL and working range from 4.02 ng/mL to 31.25 ng/mL for 1-NAP in optimized working buffer. The matrix effect of samples was eliminated via 15-fold dilution of optimized working buffer. Good average recoveries (102.4%-123.4%) with a coefficient of variation from 11.7% to 14.7% was obtained for spiked urine samples. Subsequent instrument verification test showed good correlation between the results of ciELISA and high-performance liquid chromatography. The developed ciELISA is a high-throughput tool to monitor 1-NAP in urine, which can provide technical support for the establishment of biological exposure level for the exposure to carbaryl, naphthalene and other related pollutants.
Authors: B Marczynski; R Preuss; T Mensing; J Angerer; A Seidel; A El Mourabit; M Wilhelm; T Brüning Journal: Int Arch Occup Environ Health Date: 2005-02-22 Impact factor: 3.015
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