David Greenwood1, Beral Afacan2, Gulnur Emingil3, Nagihan Bostanci1, Georgios N Belibasakis1. 1. Division of Oral Diseases, Department of Dental Medicine , Karolinska Institutet, Huddinge, 14104, Sweden. 2. Department of Periodontology, School of Dentistry, Adnan Menderes University, Aydin, 09100, Turkey. 3. Department of Periodontology, School of Dentistry, Ege University, İzmir, 35100, Turkey.
Abstract
PURPOSE: Periodontitis is linked to a localized dysbiotic microbial shift. This trending may often not be evident due to deep taxonomic changes of low abundance organisms and lack of consideration of variations in the treatment response. By using next generation sequencing this study aims to evaluate the salivary microbiome dynamics of periodontal treatment and the implication of treatment outcome EXPERIMENTAL DESIGN: Patients with generalized aggressive periodontitis are treated non-surgically and followed up for 6 months. Saliva is collected for microbiome profiling by next generation sequencing and diversity analysis, as well as quantitative real-time polymerase chain reaction (qPCR). The treatment outcome on the first follow-up is also considered. RESULTS: Clinical parameters are significantly improved following treatment, but with no accompanying relative abundance changes on the phylum, genus and species levels, or diversity indices. Distinctive differences are observed on species level when the sensitive qPCR is used. Patients responding poorly to treatment display a marginally lower microbiome profile distance from baseline, compared to those responding favorably. CONCLUSION AND CLINICAL RELEVANCE: Periodontal treatment does not alter the broader salivary microbiome profile, but may have selective implications on the species level. Treatment outcome can be impactful in the microbiome profile, as reduced microbiome changes may be associated with poorer clinical responses.
PURPOSE:Periodontitis is linked to a localized dysbiotic microbial shift. This trending may often not be evident due to deep taxonomic changes of low abundance organisms and lack of consideration of variations in the treatment response. By using next generation sequencing this study aims to evaluate the salivary microbiome dynamics of periodontal treatment and the implication of treatment outcome EXPERIMENTAL DESIGN:Patients with generalized aggressive periodontitis are treated non-surgically and followed up for 6 months. Saliva is collected for microbiome profiling by next generation sequencing and diversity analysis, as well as quantitative real-time polymerase chain reaction (qPCR). The treatment outcome on the first follow-up is also considered. RESULTS: Clinical parameters are significantly improved following treatment, but with no accompanying relative abundance changes on the phylum, genus and species levels, or diversity indices. Distinctive differences are observed on species level when the sensitive qPCR is used. Patients responding poorly to treatment display a marginally lower microbiome profile distance from baseline, compared to those responding favorably. CONCLUSION AND CLINICAL RELEVANCE: Periodontal treatment does not alter the broader salivary microbiome profile, but may have selective implications on the species level. Treatment outcome can be impactful in the microbiome profile, as reduced microbiome changes may be associated with poorer clinical responses.
Authors: Pune N Paqué; Christopher Herz; Daniel B Wiedemeier; Konstantinos Mitsakakis; Thomas Attin; Kai Bao; Georgios N Belibasakis; John P Hays; Joël S Jenzer; Wendy E Kaman; Michal Karpíšek; Philipp Körner; Johannes R Peham; Patrick R Schmidlin; Thomas Thurnheer; Florian J Wegehaupt; Nagihan Bostanci Journal: J Pers Med Date: 2021-03-23
Authors: Ana Soriano-Lerma; Virginia Pérez-Carrasco; Manuel Sánchez-Marañón; Matilde Ortiz-González; Victoria Sánchez-Martín; Juan Gijón; José María Navarro-Mari; José Antonio García-Salcedo; Miguel Soriano Journal: Sci Rep Date: 2020-08-12 Impact factor: 4.379
Authors: Armelia Sari Widyarman; Citra Fragrantia Theodorea; Nadeeka S Udawatte; Aradhea Monica Drestia; Endang W Bachtiar; Tri Erri Astoeti; Boy M Bachtiar Journal: Front Oral Health Date: 2021-11-29