Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Rapid step-gradient purification of mitochondrial DNA.

Literature DB >> 3221842

Rapid step-gradient purification of mitochondrial DNA.

Abstract

A convenient modification of the step gradient (CsCl/ethidium bomide) procedure is described. This rapid method allows isolation of covalently closed circular DNA separated from contaminating proteins, RNA and chromosomal DNA in ca. 5 h. Large scale preparations can be performed for circular DNA from eukaryotic organelles (mitochondria). The protocol uses organelle pelleting/NaCl-sarcosyl incubation steps for mitochondria followed by a CsCl step gradient and exhibits yields equal to the conventional procedures. It results in DNA sufficiently pure to be used for restriction endonuclease analysis, subcloning, 5'-end labeling, gel retention assays, and various types of hybridization.

Entities: Chemical

Mesh：

Substances：

Year: 1988 PMID： 3221842 DOI： 10.1007/bf00539059

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

14 in total

1. Purification of RNA-free plasmid DNA using alkaline extraction followed by Ultrogel A2 column chromatography.

Authors: D Micard; M L Sobrier; J L Couderc; B Dastugue
Journal: Anal Biochem Date: 1985-07 Impact factor: 3.365

2. Mapping of the displacement loop within the nucleotide sequence of Xenopus laevis mitochondrial DNA.

Authors: S S Cairns; D F Bogenhagen
Journal: J Biol Chem Date: 1986-06-25 Impact factor: 5.157

3. A new rapid procedure for the preparation of plasmid DNA.

Authors: P A Edwardson; T Atkinson; C R Lowe; D A Small
Journal: Anal Biochem Date: 1986-02-01 Impact factor: 3.365

4. The c-erb-A protein is a high-affinity receptor for thyroid hormone.

Authors: J Sap; A Muñoz; K Damm; Y Goldberg; J Ghysdael; A Leutz; H Beug; B Vennström
Journal: Nature Date: 1986 Dec 18-31 Impact factor: 49.962

5. A rapid boiling method for the preparation of bacterial plasmids.

Authors: D S Holmes; M Quigley
Journal: Anal Biochem Date: 1981-06 Impact factor: 3.365

Review 6. Human chromosome 22.

Authors: J C Kaplan; A Aurias; C Julier; M Prieur; M F Szajnert
Journal: J Med Genet Date: 1987-02 Impact factor: 6.318

7. Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis.

Authors: W M Brown
Journal: Proc Natl Acad Sci U S A Date: 1980-06 Impact factor: 11.205

8. A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.

Authors: R Schneider; I Gander; U Müller; R Mertz; E L Winnacker
Journal: Nucleic Acids Res Date: 1986-02-11 Impact factor: 16.971

Rapid step-gradient purification of mitochondrial DNA.

1. Purification of RNA-free plasmid DNA using alkaline extraction followed by Ultrogel A2 column chromatography.

2. Mapping of the displacement loop within the nucleotide sequence of Xenopus laevis mitochondrial DNA.

3. A new rapid procedure for the preparation of plasmid DNA.

4. The c-erb-A protein is a high-affinity receptor for thyroid hormone.

5. A rapid boiling method for the preparation of bacterial plasmids.

Review 6. Human chromosome 22.

7. Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis.

8. A sensitive and rapid gel retention assay for nuclear factor I and other DNA-binding proteins in crude nuclear extracts.

9. Rapid purification of plasmid DNA by a single centrifugation in a two-step cesium chloride-ethidium bromide gradient.

10. Characterization of the supernumerary chromosome in cat eye syndrome.

1. DNA curvature in front of the human mitochondrial L-strand replication origin with specific protein binding.

2. Renal oncocytoma. A phenotypic and genotypic entity of renal parenchymal tumors.

Review 3. Mitoproteomics: Tackling Mitochondrial Dysfunction in Human Disease.