Impact of the Activation Status of the Akt/mTOR Signalling Pathway on the Clinical Behaviour of Synovial Sarcoma: Retrospective Analysis of 174 Patients at a Single Institution.

PURPOSE: Phosphoinositide 3-kinase (PI3K) and the downstream Akt/mammalian target of rapamycin (mTOR) pathway are central to the control of cell proliferation and survival. Although abnormal activation of this pathway has been well established in a variety of tumours, limited studies are available on synovial sarcoma. The aim of this study was to investigate the expression of several key proteins of those pathways in synovial sarcomas and to correlate the expression of these proteins with clinicopathologic features and prognosis. PATIENTS AND METHODS: A total of 174 patients with synovial sarcomas were recruited for this study. The phosphorylation status of Akt, mTOR, and eukaryotic translation initiation factor 4E binding protein (4E-BP1) was measured by immunohistochemistry assays in formalin-fixed, paraffin-embedded samples. Correlations between the expression levels of these proteins and clinicopathologic features and prognosis were analysed.
RESULTS: The positive rates of phosphorylated (p)Akt, pmTOR, p4E-BP1, and CyclinD1 were 62.7%, 55.6%, 47.1%, and 52.6%, respectively. The positive results of pmTOR, pAkt, and downstream p4E-BP1 were correlated with each other. The positive pAkt, pmTOR, p4E-BP1, and CyclinD1 results were more highly expressed in head and neck and visceral tumours, and positive p4E-BP1 results were correlated with larger size and larger areas of necrosis. In multivariate analysis of clinicopathologic factors, head and neck and visceral location, large tumour size, larger areas of necrosis and frequent mitosis were confirmed as risk factors for shorter overall survival. Positive pAkt, pmTOR and p4E-BP1 results were correlated significantly with shorter overall survival, and CyclinD1 was not in the univariate analysis. The positive pmTOR, pAkt, p4E-BP1, and CyclinD1 results were significantly poor prognostic factors for overall survival, and only positive p4E-BP1 results were significantly associated with shorter event-free survival in multivariate analysis.
CONCLUSION: This study demonstrated the high expression of pAkt, pmTOR, and p4E-BP1 associated with aggressive clinical behaviour in synovial sarcomas and provided evidence for prognostic evaluation and targeted therapy.

Introduction

Synovial sarcoma (SS) is a translocation-associated mesenchymal malignant tumour that accounts for approximately 10% of all soft tissue sarcomas. The characteristic chromosomal translocation t (18; X) (p11; q11), which leads to the formation of a fusion protein, is thought to be the cause of this disease.1–3 Three main histological subtypes of SS have been recognised: biphasic, monophasic and poorly differentiated subtypes.4 The most common treatment for SS is wide surgical resection with or without chemotherapy and radiotherapy.5 Even with multimodal treatments, the prognosis is still poor once distant metastasis occurs.6,7 Therefore, it is important to investigate the prognostic indicator of disease progression and overall survival in SS.

The phosphoinositide 3-kinase (PI3K) and the downstream Akt/mammalian target of rapamycin (mTOR) pathway are central to the control of cell transcription, translation, metabolism, proliferation, migration and survival.8 Preclinical and epidemiological studies have confirmed that the PI3K/AKT/mTOR pathway plays an essential role in tumour progression and that it is a pivotal factor in regulating tumour cell metabolism and tumour angiogenesis.9,10 Although abnormal activation of the PI3K/Akt/mTOR pathway has been well established in a variety of tumours, including lung cancer, colorectal cancer, breast cancer, endometrial carcinoma and glioblastoma, limited studies are available in synovial sarcoma.11–15 CyclinD1 is a key regulator of the G1/S transition of the cell cycle and is overexpressed in a variety of malignancies. Umekita Y and Ikehara M showed that overexpression of CyclinD1 predicted poor prognosis in breast cancer and adenocarcinomas of the lung.16,17 J Averous observed that 4E-BP1 played a key role in coupling CyclinD1 expression to mTORC1 signalling.18 In this study, we focused on the phosphorylation status of Akt, mTOR, 4E-BP1, and CyclinD1 in a large series of synovial sarcomas and then analysed the relationship of pAkt, pmTOR, p4E-BP1, and CyclinD1 with clinicopathologic features and prognosis.

Materials and Methods

Patients and Materials

We collected 554 consecutive cases from the database of the Department of Pathology, Chinese PLA General Hospital, with a histological diagnosis of synovial sarcoma who were treated between January 2006 and December 2016. Pathology reports and clinical charts were reviewed, and all the useful information was collected. In order to avoid the diagnosis deviation from SS, sections of all patients were re-evaluated independently by two experienced pathologists (Huai-Yin Shi and Hong-Qun Wang). When required, a molecular confirmation was performed by fluorescence in situ hybridisation (FISH) or reverse transcription polymerase chain reaction (RT-PCR) to detect the presence of the specific (X;18)(p11;q11) translocations. The material for the immunohistochemistry was inadequate in 318 cases, and data on outcome were incomplete in 52 cases; therefore, only 174 patients could be included in this study and retrospectively analysed for survival.

Written informed consent was obtained from all patients or their legal guardians and with ethics committee approval of Chinese PLA General Hospital (NO. S2019-314-01). This study was conducted in accordance with the Declaration of Helsinki.

Tumour location was classified as head and neck, distal extremities, proximal extremities and trunk or viscera. Tumours were classified histologically into biphasic, monophasic, or poorly differentiated by pathologists using the World Health Organization (WHO) classification. The French Federation of Cancer Centers (FNCLCC) grading system was used to evaluate the extent of necrosis and mitosis.

Immunohistochemistry

The immunohistochemical study was performed for phospho-Akt (pAkt) (Ser473) (D9E) XPRabbit mAb (Cell Signaling Technology), rabbit anti-mTOR (phospho S2448) antibody (pmTOR) (Abcam), phospho-4E-BP1 (p4E-BP1) (Thr37/46) (236B4) (Cell Signaling Technology), CyclinD1 (clone DSC6, DAKO) and the mouse monoclonal antibody for Ki-67 (MIB-1) (DAKO). Four-micrometre-thick sections of formalin-fixed paraffin-embedded samples were stained according to laboratory standard operating procedures. The sections were pre-treated using pressure cooker antigen retrieval (citrate buffer; pH 6.0) for 2 min, using pAkt (1:50 dilution), pmTOR (1:400 dilution), p4E-BP1 (1:400 dilution), CyclinD1 (1:300 dilution) and Ki-67 (1:100 dilution) as primary antibodies and the EnVision Plus detection system (DAKO).19 Immunostaining of pAkt, pmTOR and p4E-BP1 was recognised in both the cytoplasm and nuclei, and staining of CyclinD1 and Ki-67 showed a nuclear staining pattern. The staining intensity and percentage of positive tumour cells were evaluated by two independent pathologists, and the cases were counted as “positive” or “high expression” when >10% of tumour cells showed moderate or strong staining intensity. PBS was used instead of the primary antibody to serve as a negative control, and slides of tissues known to express pAkt, pmTOR, p4E-BP1, CyclinD1 and Ki-67 were used as positive controls in each staining.

Statistical Analysis

All statistical analyses were performed using SPSS statistical software (version 17.0, Inc., Chicago). Associations between clinicopathologic factors and immunohistochemical staining results for pmTOR, pAkt, p4E-BP1, and CyclinD1 were analysed with the χ2 test or Fisher’s exact test. Overall survival (OS) and event-free survival (EFS) were estimated using the Kaplan-Meier method. OS time was defined as the time between histologic diagnosis and death or the last follow-up visit. EFS time was defined as the time between histologic diagnosis and the local recurrence, distant recurrence, or death. Correlations between clinicopathologic factors and immunopositivity for pAkt, pmTOR, and p4E-BP1 with survival were analysed by the Log rank test. Multivariable analysis was performed using Cox regression models. P values < 0.05 were considered statistically significant.

Results

Patients and Tumour Clinicopathologic Parameters

The clinicopathologic parameters and the survival analysis results of all 174 patients are summarised in Table 1 and Figures 1 and 2. The follow-up ranged from 10 months to 231 months (median, 63 months) for OS in 174 patients with an OS rate of 68.4%. The follow-up ranged from 1 month to 195 months (median, 24 months) for EFS in 174 patients with a 5-year EFS rate of 40.5%. Forty-eight percent of synovial sarcoma occurred in the proximal extremities, with significant differences in tumour location between the <30 age group and the ≥30 age group (P = 0.0027). Proximal tumours were more likely to undergo chemotherapy (P < 0.001) and radiation (P = 0.0004) than were other tumours, and younger age was significantly associated with chemotherapy (<30 are group 79.37%, ≥30 age group 64.86%) (P = 0.0277). In terms of surgical margin, wide resection was adopted for distal and proximal tumours, while marginal resection or intralesional resection was more common for head and neck and visceral tumours (P < 0.001). Large tumours tended to adopt wide resection (P = 0.006). Poorly differentiated SS exhibited larger areas of necrosis (P < 0.001) and more frequent mitosis (P = 0.007) than those of the other (biphasic or monophasic) histologic subtypes. Visceral tumours had larger areas of necrosis (P = 0.046) and more frequent mitosis (P = 0.038) than those of the other (head and neck, distal extremities, proximal extremities and trunk) locations (data not shown).

Table 1

Clinicopathologic Parameters and Survival Analysis

	N (%)	5-Year EFS, %	P	5-Year OS, %	P	Analysed Groups
Sex			0.161		0.701
Male	90	35.1		74.8
Female	84	46.5		81.6
Age			0.423		0.917
<30	63	34.0		78.9
≥30	111	60.8		77.3
Location			< 0.001a		< 0.001a
Distal extremities	51	53.3		88.3
Proximal extremities and trunk	84	51.5		94.2
Viscera	23	6.6		42.2
Head and neck	16	0		23.4
Size (cm)			0.382		0.045a
≤5	57	40.9		82.9
>5	87	40.2		74.7
Histologic subtype			0.587		0.102
Mono	106	39.3	0.348	78.4	0.986	Mono vs Bi
Bi	41	43.1	0.600	77.9	0.029a	Poor vs Mono
Poor	12	52.1	0.835	70.0	0.138	Poor vs Bi
NA	15
Necrosis			0.225		0.039a
Score 0, none	27	50.7	0.133	85.6	0.348	Score 0 vs Score 1
Score 1, ≤50%	93	35.9	0.810	81.3	0.022a	Score 0 vs Score 2
Score 2, >50%	29	46.2	0.286	61.3	0.042a	Score 1 vs Score 2
NA	25
Mitosis			0.156		< 0.001a
Score 1, 0–9/10 HPF	83	44.1	0.765	82.4	0.089	Score 1 vs Score 2
Score 2, 10–19/10 HPF	27	51.6	0.060	92.6	< 0.001a	Score 1 vs Score 3
Score 3, ≥20/10 HPF	40	20.2	0.143	57.7	0.041a	Score 2 vs Score 3
NA	30
Ki-67			0.587		0.761
≤10%	29	47.6		75.5
>10%	87	42.6		77.5
NA	58
Chemotherapy			0.150		< 0.001a
YES	122	44.4		89.9
NO	52	30.3		48.6
Radiation			0.969		0.212
YES	59	42.9		87.2
NO	115	40.0		73.8
Surgical margin			< 0.001a		< 0.001a
Wide	114	54.8		90.5
Marginal	38	15.4		74.4
Intralesional	22	11.1		21.5

Note: aStatistically significant.

Abbreviations: Bi, biphasic synovial sarcoma; EFS, event-free survival; HPF, high-power field; Mono, monophasic synovial sarcoma; NA, not available; OS, overall survival; Poor, poorly differentiated synovial sarcoma.

Figure 1

Overall survival according to representative clinicopathologic parameters (P < 0.05; log-rank test).

Figure 2

Event-free survival according to location and surgical margin (P < 0.05; log-rank test).

Head and neck and visceral tumours (P < 0.001), large tumour size (P = 0.045), larger areas of necrosis (P = 0.039) and more frequent mitosis (P < 0.001) were factors found to be significantly associated with poor overall survival in patients by univariate analysis. In addition, head and neck and visceral tumours were more prone to relapse or metastasis (EFS, P < 0.001). We did not observe a different prognosis according to the histologic subtype (EFS, P = 0.587; OS, P = 0.102).

Chemotherapy, Radiation and Surgical Margin

One hundred fifteen patients received adjuvant chemotherapy (74 preoperative and 41 postoperative). The most common adjuvant chemotherapy regimens were a combination of ifosfamide (at a dose of 9 g/m2) and doxorubicin (at a dose of 80 mg/m2) or epirubicin (at a dose of 120 mg/m2). The patients who had received adjuvant chemotherapy (preoperative and/or postoperative) had a better prognosis than those without chemotherapy (OS, P < 0.001; EFS, P = 0.068). Furthermore, patients who received more than three preoperative chemotherapy sessions had a better prognosis than patients with only postoperative chemotherapy (OS, P = 0.019; EFS, P = 0.036) (data not shown). There was no significant difference in the use of radiation. In terms of surgical margin, the prognosis of patients undergoing extensive tumour resection was significantly better than that of patients undergoing marginal resection or internal resection (OS, P < 0.001; EFS, P < 0.001).

Immunohistochemical Reactivity of pmTOR, pAkt, p4E-BP1, and CyclinD1

The results of immunohistochemical studies of pmTOR, pAkt, p4E-BP1, and CyclinD1 are summarised in Table 2 and in Figure 3. The positive rates of pmTOR, pAkt, p4E-BP1, and CyclinD1 were 62.7%, 55.6%, 47.1%, and 52.6%, respectively. The expression of pmTOR, pAkt, and p4E-BP1 was mainly cytoplasmic, and in some cases, parallel nuclear staining was visible. In the tumours of biphasic SS, much stronger staining in nuclei than that in the cytoplasm of epithelioid tumour cells was observed, and the positive rate of epithelioid-predominant staining pattern was pAkt (62.5%, 10/16, P = 0.359), pmTOR (70.0%, 7/10, P = 0.559) and p4E-BP1 (53.3%, 8/15, P = 0.013). In poorly differentiated SS, the positive rates of pAkt, pmTOR and p4E-BP1 were likely to be higher than those of other tissue subtypes and were 80.0% (4/5, P = 0.359), 80.0% (4/5, P = 0.559) and 90% (9/10, P = 0.013), respectively. CyclinD1 expression was nuclear and displayed an epithelioid-predominant staining pattern in biphasic SS. The positive rate of CyclinD1 in monophasic, biphasic and poorly differentiated SS was 45%, 84.6%, and 50%, respectively (P = 0.030).

Table 2

Immunohistochemical Results and Survival Analysis

	N(%)	5-Year EFS, %	P	5-Year OS, %	P
pAkt			0.093		0.029a
(＋)	35(55.6)	28.9		75.0
(－)	28(44.4)	47.0		92.0
pmTOR			0.124		0.032a
(＋)	32(62.7)	30.7		70.2
(－)	19(37.3)	56.1		93.3
p4E-BP1			0.857		0.023a
(＋)	56(47.1)	39.1		70.4
(－)	63(52.9)	37.1		87.8
CyclinD1			0.109		0.934
(＋)	30(52.6)	39.9		73.1
(－)	27(47.4)	34.4		80.0

Note: aStatistically significant.

Abbreviations: EFS, event-free survival; OS, overall survival; p4E-BP1, phosphorylated eukaryotic translation initiation factor 4E-binding protein; pAkt, phosphorylated protein kinase B; pmTOR, phosphorylated mammalian target of rapamycin.

Figure 3

The results of immunohistochemistry are shown, indicating immunopositivity for (A) phosphorylated protein kinase B (pAkt), (B) phosphorylated mammalian target of rapamycin (pmTOR), (C) phosphorylated eukaryotic translation initiation factor 4E-binding protein (p4E-BP1), and (D) CyclinD1.

The statistical analysis for possible correlations of the immunohistochemical results and clinicopathologic parameters was performed, showing several significant associations (Table 3). The positive results of pmTOR, pAkt, and downstream p4E-BP1 were correlated with each other, whereas CyclinD1 expression did not show a significant correlation with other molecules in the Akt/mTOR signalling pathway. Tumours with pAKT expression usually showed pmTOR positivity (P < 0.001) and p4E-BP1 (P < 0.001) positivity. Furthermore, pmTOR positivity was significantly associated with p4E-BP1 positivity (P = 0.038). pAkt, pmTOR, p4E-BP1, and CyclinD1 were more highly expressed in head and neck and visceral tumours than in distal extremities, proximal extremities and the trunk (P = 0.042, P = 0.027, and P = 0.025, respectively). There was no difference in the expression of pAkt, pmTOR and p4E-BP1 between the preoperative chemotherapy group and the postoperative chemotherapy group, but the expression of CyclinD1 in the preoperative chemotherapy group was lower than that in the postoperative chemotherapy group (P = 0.013). The positive results of p4E-BP1 tended to occur in larger size tumours (P = 0.026) and in those with larger areas of necrosis (P = 0.023).

Table 3

Statistical Analysis of the Immunohistochemical Results and Clinicopathologic Parameters

	pAkt	pmTOR	p4E-BP1	CyclinD1
pAkt	–	–	–	–
pmTOR	< 0.001a	–	–	–
p4E-BP1	< 0.001a	0.038a	–	–
CyclinD1	0.237	0.114	0.342	–
Sex	0.866	0.180	0.762	0.088
Age	0.728	0.232	0.826	0.439
Location	0.042aP vs V, H	0.027aP vs H	0.025a	0.059
Size (cm)	0.586	0.529	0.026a	0.465
Histologic subtype	0.359	0.559	0.013a	0.030a
Necrosis	0.197	0.615	0.073	0.265
Mitosis	0.165	0.807	0.229	0.517
Ki-67	0.869	0.286	0.947	0.655
Chemotherapy	0.124	0.232	0.208	0.013a

Note: aStatistically significant.

Abbreviations: H, head and neck; P, proximal extremities and trunk; p4E-BP1, phosphorylated eukaryotic translation initiation factor 4E-binding protein; pAkt, phosphorylated protein kinase B; pmTOR, phosphorylated mammalian target of rapamycin; V, viscera.

The survival analysis of the immunohistochemical results is summarised in Table 2 and in Figure 4. Immunopositivity of pAkt, pmTOR and p4E-BP1 was a significant risk factor for a poorer prognosis (OS: P = 0.042, P = 0.027, and P = 0.025, respectively). The Kaplan-Meier survival curves for overall survival rates according to the immunohistochemical results of pAkt, pmTOR and p4E-BP1 are displayed in Figure 4. We did not observe a different prognosis according to the immunohistochemical results of CyclinD1.

Figure 4

Overall survival according to the results from immunohistochemical studies of (A) phosphorylated Akt (pAkt), (B) phosphorylated mammalian target of rapamycin (pmTOR), and (C) phosphorylated eukaryotic translation initiation factor 4E-binding protein (p4E-BP1).

Multivariate Survival Analysis

After multivariate analysis of clinicopathologic factors, head and neck and visceral location, large tumour size, larger areas of necrosis and frequent mitosis were confirmed as negative prognostic parameters. The immunohistochemical results of pmTOR, pAkt, p4E-BP1, and CyclinD1 were adjusted by the above 4 clinicopathologic parameters (location, size, tumour necrosis and mitotic activity) (Table 4), and the multivariate analysis indicated that pmTOR, pAkt, p4E-BP1, and CyclinD1 were significantly poor prognostic factors for overall survival (P < 0.001, P = 0.001, P < 0.001, and P < 0.001, respectively), and only positive p4E-BP1 results were significantly associated with shorter event-free survival (P = 0.034).

Table 4

Multivariate Survival Analysis for Immunohistochemical Parameters Adjusted by Location, Size, Tumour Necrosis and Mitotic Activity

Parameters	Multivariate Survival Analysis: P
	EFS	OS
pAkt	0.562	< 0.001a
pmTOR	0.728	0.001a
p4E-BP1	0.034a	< 0.001a
CyclinD1	0.097	< 0.001a

Note: aStatistically significant.

Abbreviations: EFS, event-free survival; OS, overall survival; p4E-BP1, phosphorylated eukaryotic translation initiation factor 4E-binding protein; pAkt, phosphorylated protein kinase B; pmTOR, phosphorylated mammalian target of rapamycin.

Discussion

To develop effective targeted therapies, several key signalling pathways have attracted interest over the past decade and have been identified as potential treatment targets in sarcoma.1,20 The Akt/mTOR pathway regulates all critical phases of cell growth, including proliferation, differentiation, and apoptosis, and deregulation of this pathway has been reported in several types of tumours.8,13,14,21,22 The oncogenic role of aberrant Akt/mTOR signalling pathway activation has been investigated in sarcoma.11,23 However, few previous studies from the Chinese population have focused on synovial sarcomas. The present study aimed to investigate the mechanism of activation of the Akt/mTOR pathway and its engagement in clinicopathologic features and prognosis in a large series of synovial sarcomas from a Chinese population.

Several adverse clinical prognostic factors of synovial sarcomas have been identified in previous large studies.6,7,24–27 The results of our study demonstrated that large tumour size, head and neck and visceral location, larger areas of necrosis and frequent mitosis were poor prognostic factors for SS. Large tumour size, larger areas of necrosis and high mitotic activity have been consistently associated with poor outcomes for SS in many previous studies.6,28,29 We also observed that head and neck and visceral tumours were more prone to relapse or metastasis. As far as we know, the prognostic implications of head and neck and visceral location have rarely been assessed; however, Deshmukh et al reported that proximal and truncal tumours have a worse survival than tumours of the distal extremities.29 G. Louis et al reported that small and superficial SSs have a better overall survival than larger and deeper-seated synovial sarcomas.30 The role of the histologic subtype was not certain; biphasic histology appears to be associated with better prognoses, but in our study, we demonstrated a statistically significant difference in overall survival when we compared monophasic synovial sarcoma with poorly differentiated synovial sarcoma.

Whether chemotherapy provides a clinically meaningful benefit for patients with SS with localised disease is still debated. In our study, the patients who had received adjuvant chemotherapy (preoperative and/or postoperative) had a better prognosis than those without chemotherapy. Furthermore, patients who received more than three preoperative chemotherapy sessions had a better prognosis than patients with only postoperative chemotherapy. Unlike our SS studies, the current series did not demonstrate better survival for patients with localised disease undergoing chemotherapy.25 In addition, we were not able to further verify the potential role of radiotherapy in SS. Several reports have indicated that adequate surgical excision is the mainstay of treatment for SS.31 We also found that the prognosis of patients undergoing extensive tumour resection was significantly better than that of patients undergoing marginal resection or internal resection.

The phosphorylation of kinases along the Akt/mTOR pathway reflects their activated status. In our study, we used immunohistochemistry with three phosphorylation-specific antibodies to detect Akt, mTOR, and 4E-BP1 phosphorylation status in synovial sarcomas. We found that higher levels of pAKT, pmTOR, and p4E-BP1 were statistically significantly associated with poor OS. The immunopositivity for pAkt, pmTOR, and p4E-BP1 was identified as an adverse prognostic factor in this study. These results support previous studies on patients with gastric carcinomas, breast cancers and sarcomas.23,26,32 We also found that each pair of phosphorylated proteins was highly positively associated in synovial sarcomas, ie, tumours with pAKT expression usually showed pmTOR positivity and p4E-BP1 positivity. Furthermore, pmTOR positivity was significantly associated with p4E-BP1 positivity. This phenomenon confirmed the activation cascade of the Akt/mTOR/4E-BP1 pathway in synovial sarcomas. In previous studies, Zhou et al also reported that the positive expression results of pmTOR, pAkt, and downstream p4E-BP1 were correlated with each other in invasive breast cancers.33 Although we did not observe a different prognosis according to the immunohistochemical results of CyclinD1, the multivariate analysis indicated that CyclinD1 was a significantly poor prognostic factor for overall survival. In contrast, EWA et al reported that pAKT expression was associated with high CyclinD1 labelling in neuroblastoma,21 whereas CyclinD1 expression did not show a significant correlation with other molecules in the Akt/mTOR signalling pathway in our study.

In poorly differentiated SS, the positive rate of pAkt, pmTOR and p4E-BP1 was likely to be higher than that in other tissue subtypes. pAkt, pmTOR, and p4E-BP1 were more highly expressed in head and neck and visceral tumours than in distal extremities, proximal extremities and the trunk. Kazuki et al reported that pAkt tended to increase with low differentiation and deep invasion in oral cancer.34 These features suggested that pAkt, pmTOR and p4E-BP1 might correlate with low differentiation and could be prognostic factors. The univariate prognostic factor p4E-BP1 was correlated with a larger size and a larger extent of necrosis. Because p4E-BP1 is considered a funnelling factor through which the transforming signals converge, it channels the oncogenic proliferative signalling pathways regardless of the upstream specific oncogenic alteration. EWA et al reported that high expression of p-4E-BP1 was significantly associated with lower tumour differentiation.21

In our study, we demonstrated that the expression of pmTOR, pAkt, and p4E-BP1 was mainly cytoplasmic, and in some cases, parallel nuclear staining was visible. Kazuki et al showed that pAkt and pmTOR were localised to the nuclei and cytoplasm of epithelial or carcinoma cells in oral squamous cell carcinoma.34 EWA et al observed concurrent cytoplasmic and nuclear pmTOR expression in neuroblastoma and p4E-BP1 in most cases of neuroblastoma in the cytoplasm and often within the nucleus.21 In addition, we found that much stronger staining in nuclei than in cytoplasm of epithelioid tumour cells was observed in the biphasic SS tumours. Setsu et al have also reported that some tumours had extensive staining in the cytoplasm of epithelioid SS cells.23 This epithelioid-dominant staining pattern was reported in some previous studies, but its significance remains unclear.35

As a downstream target of phosphorylated Akt, inhibition of mTOR would also be a potential therapeutic approach to reduce the effects of constitutively activated Akt in sarcomas. Previous studies have demonstrated that mTOR inhibition could decrease the proliferation, migration and invasion of multiple synovial sarcoma cell lines in vitro.36 Moreover, clinical trials of molecular targeting drugs have also been undertaken in soft-tissue sarcoma. Ridaforolimus, a small-molecule kinase inhibitor of mTOR, exhibited significant antitumor activity in preclinical and clinical studies and prolonged progression-free survival in a clinical trial targeting advanced soft-tissue sarcoma, although there was no significant difference in OS.37,38 Pazopanib, a multitarget kinase inhibitor, also exhibited potential efficacy against soft-tissue sarcoma, especially in a group of patients with SS.39,40 In our study, frequent deregulation of the Akt/mTOR signalling pathway and its prognostic role in synovial sarcomas support the notion of using mTOR inhibitors as an additional synovial sarcoma treatment. Moreover, the detection of pAkt, pmTOR, or p4E-BP1 through the simplicity and reproducibility of immunohistochemical staining might be very helpful for identifying and predicting which patients are most likely to derive the most benefit from treatment with an mTOR inhibitor.

Conclusion

We analysed the expression of key proteins of the Akt/mTOR pathway, including pAkt, pmTOR, and p4E-BP1, in synovial sarcomas, and this was compared to relevant clinicopathologic features and prognosis of synovial sarcomas. We demonstrated the high expression of pAkt, pmTOR, and p4E-BP1 associated with aggressive clinical behaviour in SS. Further study of the Akt/mTOR pathway will be helpful in determining better markers for prognostic evaluation and effective therapeutic targets for synovial sarcomas.