Literature DB >> 32194931

A novel workflow for three-dimensional analysis of tumour cell migration.

Sophie Ketchen1, Arndt Rohwedder2, Sabine Knipp2, Filomena Esteves1, Nina Struve3, Michelle Peckham2, John E Ladbury2, Alistair Curd2, Susan C Short1, Anke Brüning-Richardson4.   

Abstract

The limitations of two-dimensional analysis in three-dimensional (3D) cellular imaging impair the accuracy of research findings in biological studies. Here, we report a novel 3D approach to acquisition, analysis and interpretation of tumour spheroid images. Our research interest in mesenchymal-amoeboid transition led to the development of a workflow incorporating the generation and analysis of 3D data with instant structured illumination microscopy and a new ImageJ plugin.
© 2020 The Author(s).

Entities:  

Keywords:  3D; ImageJ; cell migration; microscopy; tumour spheroid

Year:  2020        PMID： 32194931      PMCID： PMC7061943          DOI： 10.1098/rsfs.2019.0070

Source DB:  PubMed          Journal:  Interface Focus        ISSN： 2042-8898            Impact factor:   3.906


  10 in total

1.  Cell migration: Moving in 3D.

Authors:  Kim Baumann
Journal:  Nat Rev Mol Cell Biol       Date:  2010-06-09       Impact factor: 94.444

Review 2.  Filopodia: molecular architecture and cellular functions.

Authors:  Pieta K Mattila; Pekka Lappalainen
Journal:  Nat Rev Mol Cell Biol       Date:  2008-05-09       Impact factor: 94.444

Review 3.  Factors associated with failure of oncology drugs in late-stage clinical development: A systematic review.

Authors:  Denis L Jardim; Eric S Groves; Philip P Breitfeld; Razelle Kurzrock
Journal:  Cancer Treat Rev       Date:  2016-11-04       Impact factor: 12.111

Review 4.  Current challenges in clinical target volume definition: tumour margins and microscopic extensions.

Authors:  Leyla Moghaddasi; Eva Bezak; Loredana G Marcu
Journal:  Acta Oncol       Date:  2012-09-24       Impact factor: 4.089

5.  In vitro evaluation of clinical activity and toxicity of anticancer drugs using tumor cells from patients and cells representing normal tissues.

Authors:  Caroline Haglund; Anna Aleskog; Peter Nygren; Joachim Gullbo; Martin Höglund; Malin Wickström; Rolf Larsson; Elin Lindhagen
Journal:  Cancer Chemother Pharmacol       Date:  2011-10-09       Impact factor: 3.333

6.  CCG-1423: a small-molecule inhibitor of RhoA transcriptional signaling.

Authors:  Chris R Evelyn; Susan M Wade; Qin Wang; Mei Wu; Jorge A Iñiguez-Lluhí; Sofia D Merajver; Richard R Neubig
Journal:  Mol Cancer Ther       Date:  2007-08       Impact factor: 6.261

Review 7.  The molecular mechanisms of transition between mesenchymal and amoeboid invasiveness in tumor cells.

Authors:  K Panková; D Rösel; M Novotný; Jan Brábek
Journal:  Cell Mol Life Sci       Date:  2009-08-26       Impact factor: 9.261

8.  High-content analysis of tumour cell invasion in three-dimensional spheroid assays.

Authors:  Vinton Cheng; Filomena Esteves; Aruna Chakrabarty; Julia Cockle; Susan Short; Anke Brüning-Richardson
Journal:  Oncoscience       Date:  2015-06-14

9.  Instant super-resolution imaging in live cells and embryos via analog image processing.

Authors:  Andrew G York; Panagiotis Chandris; Damian Dalle Nogare; Jeffrey Head; Peter Wawrzusin; Robert S Fischer; Ajay Chitnis; Hari Shroff
Journal:  Nat Methods       Date:  2013-10-06       Impact factor: 28.547

10.  Construction of an instant structured illumination microscope.

Authors:  Alistair Curd; Alexa Cleasby; Katarzyna Makowska; Andrew York; Hari Shroff; Michelle Peckham
Journal:  Methods       Date:  2015-07-22       Impact factor: 3.608
  10 in total
  3 in total

1.  'Cloudbuster': a Python-based open source application for three-dimensional reconstruction and quantification of stacked biological imaging samples.

Authors:  A Rohwedder; S Knipp; F O Esteves; M Hale; S E Ketchen; D Treanor; A Brüning-Richardson
Journal:  Interface Focus       Date:  2022-08-12       Impact factor: 4.661

2.  Composition of receptor tyrosine kinase-mediated lipid micro-domains controlled by adaptor protein interaction.

Authors:  Arndt Rohwedder; Sabine Knipp; Lee D Roberts; John E Ladbury
Journal:  Sci Rep       Date:  2021-03-17       Impact factor: 4.379

3.  Drug Resistance in Glioma Cells Induced by a Mesenchymal-Amoeboid Migratory Switch.

Authors:  Sophie E Ketchen; Filomena O Gamboa-Esteves; Sean E Lawler; Michal O Nowicki; Arndt Rohwedder; Sabine Knipp; Sally Prior; Susan C Short; John E Ladbury; Anke Brüning-Richardson
Journal:  Biomedicines       Date:  2021-12-22
  3 in total

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