Literature DB >> 321695

Improved methods for separation and purification by affinity chromatography.

G Johnson, J S Garvey.   

Abstract

Defined conditions for simplified Affinity Chromatography suitable for instructional and research purposes, were developed. The standard method of activation of polysaccharide gels using BrCN was modified and improved using a highly buffered reaction solution. A batchwise protocol for the immunoadsorbent isolation of anti-BSA was used (with commonly employed dissociation agents) and comparative data were obtained.

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Year:  1977        PMID： 321695     DOI： 10.1016/0022-1759(77)90014-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  6 in total

1.  A class capture enzyme immunoassay for immunoglobulin level determinations in bovine sera.

Authors:  K Nielsen; B Rosenbaum; J Stiller
Journal:  Can J Comp Med       Date:  1985-10

2.  Immunoglobulin G subclass antibody responses in influenza A and parainfluenza type 1 virus infections.

Authors:  I Julkunen; T Hovi; I Seppälä; O Mäkelä
Journal:  Clin Exp Immunol       Date:  1985-04       Impact factor: 4.330

3.  Bovine monoclonal antibody specific for Brucella abortus lipopolysaccharide.

Authors:  K H Nielsen; M D Henning
Journal:  Vet Immunol Immunopathol       Date:  1989-07       Impact factor: 2.046

4.  The specific in vitro antibody-mediated retention of bovine serum albumin by porcine hyaline articular cartilage.

Authors:  W P Aston; T L Ward; T D Cooke
Journal:  Clin Exp Immunol       Date:  1983-05       Impact factor: 4.330

5.  Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.

Authors:  J M Stiller; K H Nielsen
Journal:  J Clin Microbiol       Date:  1983-02       Impact factor: 5.948

6.  Alloantigen receptors on activated T cells in mice. I. Binding of alloantigens and anti-idiotypic antibodies to the same receptors.

Authors:  P H Krammer
Journal:  J Exp Med       Date:  1978-01-01       Impact factor: 14.307
  6 in total

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