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Literature DB >> 6403576

Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.

Abstract

Alkali-treated lipopolysaccharide from Brucella abortus S1119.3 coupled to agarose beads by cyanogen bromide activation resulted in an immunoadsorbent with which a large amount of B. abortus-specific antibodies could be purified. The method described gave alkali-treated lipopolysaccharide binding efficiencies of up to 98%. There was little loss of alkali-treated lipopolysaccharide from the column after several pH shifts, allowing the immunoadsorbent to be regenerated and used repeatedly.

Entities: Chemical Disease Species

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Year: 1983 PMID： 6403576 PMCID： PMC272629 DOI： 10.1128/jcm.17.2.323-326.1983

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

14 in total

1. An approach to the targeted attachment of peptides and proteins to solid supports.

Authors: K Hofmann; Y Kiso
Journal: Proc Natl Acad Sci U S A Date: 1976-10 Impact factor: 11.205

2. Coupling of Escherichia coli lipopolysaccharide to epoxy-activated Sepharose 6B.

Authors: J Fox; K Hechemy
Journal: Infect Immun Date: 1978-06 Impact factor: 3.441

3. A method of trace iodination of proteins for immunologic studies.

Authors: P J McConahey; F J Dixon
Journal: Int Arch Allergy Appl Immunol Date: 1966

4. Improved method for the cyanogen bromide activation of agarose beads.

Authors: G Kümel; H Daus; H Mauch
Journal: J Chromatogr Date: 1979-04-21

5. The serum and secretory immunoglobulins of cattle: characterization and quantitation.

Authors: J R Duncan; B N Wilkie; F Hiestand; A J Winter
Journal: J Immunol Date: 1972-04 Impact factor: 5.422

6. Binding of covalent proteins to polysaccharides by cyanogen bromide and organic cyanates. I. Preparation of soluble glycine-, insulin- and ampicillin-dextran.

Authors: L Kågedal; S Akerström
Journal: Acta Chem Scand Date: 1971

7. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors: P Cuatrecasas
Journal: J Biol Chem Date: 1970-06 Impact factor: 5.157

8. Enzyme-linked immunosorbent assay for bovine immunoglobulin subclass-specific response to Brucella abortus lipopolysaccharides.

Authors: V L Lamb; L M Jones; G G Schurig; D T Berman
Journal: Infect Immun Date: 1979-10 Impact factor: 3.441

6. Antibody-mediated protection against Brucella abortus in BALB/c mice at successive periods after infection: variation between virulent strain 2308 and attenuated vaccine strain 19.

Authors: P H Elzer; R H Jacobson; S M Jones; K H Nielsen; J T Douglas; A J Winter
Journal: Immunology Date: 1994-08 Impact factor: 7.397

7. Cleavage of bovine immunoglobulin G1 in whey by an extracellular material from Brucella abortus.

Authors: K Nielsen
Journal: Can J Comp Med Date: 1985-07

7 in total

Affinity purification of bovine antibodies to Brucella abortus Lipopolysaccharide.

1. An approach to the targeted attachment of peptides and proteins to solid supports.

2. Coupling of Escherichia coli lipopolysaccharide to epoxy-activated Sepharose 6B.

3. A method of trace iodination of proteins for immunologic studies.

4. Improved method for the cyanogen bromide activation of agarose beads.

5. The serum and secretory immunoglobulins of cattle: characterization and quantitation.

6. Binding of covalent proteins to polysaccharides by cyanogen bromide and organic cyanates. I. Preparation of soluble glycine-, insulin- and ampicillin-dextran.

7. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

8. Enzyme-linked immunosorbent assay for bovine immunoglobulin subclass-specific response to Brucella abortus lipopolysaccharides.

9. Ultracentrifugal studies of sera from cattle vaccinated or naturally infected with Brucella abortus.

10. Preparation of monospecific anti-Salmonella lipopolysaccharide antibodies by affinity chromatography.

1. Antibodies recognizing 20-hydroxyecdysone-dependent cell surface antigens during morphogenesis in Drosophila.

2. A "dipstick" enzyme immunoassay for detection of antibody to Brucella abortus in cattle sera.

3. Bovine monoclonal antibody specific for Brucella abortus lipopolysaccharide.

4. A comparison of five serological tests for bovine brucellosis.

5. Killing of Brucella abortus by bovine serum.

6. Antibody-mediated protection against Brucella abortus in BALB/c mice at successive periods after infection: variation between virulent strain 2308 and attenuated vaccine strain 19.

7. Cleavage of bovine immunoglobulin G1 in whey by an extracellular material from Brucella abortus.