Layered porous organic frameworks as a novel adsorbent for the solid phase extraction of chlorophenols prior to their determination by HPLC-DAD.

A layered porous organic framework (L-POF) with high surface area and porous volume was used as a novel adsorbent for adsorption of chlorophenols (CPs). The L-POF showed much higher adsorption capacity for four CPs (2-CP, 3-CP, 2,3-CP, 2,4-CP) compared with corresponding amorphous POF and some commercial adsorbents. Then, an L-POF-based solid-phase extraction method combined with high-performance liquid chromatography for analyzing CPs at the wavelength of 280 nm in water and honey samples was established. Under the optimum conditions, four CPs exhibited good linearity in the range 0.10-80.0 ng mL-1 for water and 2-400 ng g-1 for honey samples with determination coefficient r2 > 0.996. The method has low limits of detection of 0.03-0.10 ng mL-1 for water and 0.5-1.0 ng g-1 for honey samples. The method presented satisfactory precision and accuracy with relative standard deviations ≤ 7.8% and recoveries of 80-115%. High enrichment factors of 123-190 for water and 63-97 for honey were obtained. The validated method was successfully used to determine CPs in water and honey samples. The L-POF also displayed prominent adsorption performance for many other dyes and organic contaminants. This suggests that it can be an ideal candidate as adsorbent for wide application in separation and analysis. Graphical Abstract.