| Literature DB >> 32130900 |
Ola Grimsholm1, Eva Piano Mortari2, Alexey N Davydov3, Mikhail Shugay4, Anna S Obraztsova5, Chiara Bocci2, Emiliano Marasco6, Valentina Marcellini2, Alaitz Aranburu7, Chiara Farroni2, Domenico Alessandro Silvestris8, Cristina Cristofoletti9, Ezio Giorda2, Marco Scarsella2, Simona Cascioli2, Sabina Barresi10, Vassilios Lougaris11, Alessandro Plebani12, Caterina Cancrini13, Andrea Finocchi13, Viviana Moschese14, Diletta Valentini15, Cristina Vallone16, Fabrizio Signore16, Giovanni de Vincentiis17, Salvatore Zaffina18, Giandomenico Russo9, Angela Gallo8, Franco Locatelli19, Alberto E Tozzi20, Marco Tartaglia10, Dmitriy M Chudakov21, Rita Carsetti22.
Abstract
Memory B cells (MBCs) epitomize the adaptation of the immune system to the environment. We identify two MBC subsets in peripheral blood, CD27dull and CD27bright MBCs, whose frequency changes with age. Heavy chain variable region (VH) usage, somatic mutation frequency replacement-to-silent ratio, and CDR3 property changes, reflecting consecutive selection of highly antigen-specific, low cross-reactive antibody variants, all demonstrate that CD27dull and CD27bright MBCs represent sequential MBC developmental stages, and stringent antigen-driven pressure selects CD27dull into the CD27bright MBC pool. Dynamics of human MBCs are exploited in pregnancy, when 50% of maternal MBCs are lost and CD27dull MBCs transit to the more differentiated CD27bright stage. In the postpartum period, the maternal MBC pool is replenished by the expansion of persistent CD27dull clones. Thus, the stability and flexibility of human B cell memory is ensured by CD27dull MBCs that expand and differentiate in response to change.Entities:
Keywords: CD27; VH repertoire; aging; germinal center; immunodeficiency; immunological memory; memory B cells; pregnancy; spleen; vaccine
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Year: 2020 PMID： 32130900 DOI： 10.1016/j.celrep.2020.02.022
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423