Temoor Ahmed1,2, Muhammad Shahid2, Muhammad Noman1,2, Muhammad Bilal Khan Niazi3, Faisal Mahmood4, Irfan Manzoor2, Yang Zhang1, Bin Li1, Yong Yang5, Chengqi Yan6, Jianping Chen5,6. 1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. 2. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad 38000, Pakistan. 3. School of Chemical and Materials Engineering (SCME), National University of Sciences & Technology (NUST), Sector H-12, Islamabad 44000, Pakistan. 4. Department of Environmental Science and Engineering, Government College University, Faisalabad 38000, Pakistan. 5. State Key Laboratory for Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. 6. Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Abstract
Amongst serious biotic factors deteriorating crop yield, the most destructive pathogen of rice is Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. This study involved targeted use of biogenic silver nanoparticles (AgNPs) to control BLB in order to cope with the disadvantages of chemical disease control. AgNPs were biologically synthesized from natively isolated Bacillus cereus strain SZT1, which was identified through 16S rRNA gene sequence analysis. Synthesis of AgNPs in bacterial culture supernatant was confirmed through UV-VIS spectroscopy. Fourier transform infrared spectroscopy (FTIR) confirmed that the existence of AgNPs was stabilized with proteins and alcoholic groups. X-ray diffraction (XRD) data revealed the crystalline nature and imaging with scanning electron microscopy (SEM) and transmission electron microscopy (TEM), showing the spherical shape of AgNPs with particle sizes ranging from 18 to 39 nm. The silver presence in AgNPs was further confirmed by energy dispersive spectra. Biogenic AgNPs showed substantial antibacterial activity (24.21 ± 1.01 mm) for Xoo. In a pot experiment, AgNPs were found to be effective weapons for BLB by significantly increasing the plant biomass with a decreased cellular concentration of reactive oxygen species and increased concentration of antioxidant enzyme activity.
Amongst serious biotin class="Chemical">c fan class="Chemical">ctors deteriorating crop yield, the most destructive pathogen of rice is Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. This study involved targeted use of biogenicsilver nanoparticles (AgNPs) to control BLB in order to cope with the disadvantages of chemical disease control. AgNPs were biologically synthesized from natively isolated Bacillus cereus strain SZT1, which was identified through 16S rRNA gene sequence analysis. Synthesis of AgNPs in bacterial culture supernatant was confirmed through UV-VIS spectroscopy. Fourier transform infrared spectroscopy (FTIR) confirmed that the existence of AgNPs was stabilized with proteins and alcoholic groups. X-ray diffraction (XRD) data revealed the crystalline nature and imaging with scanning electron microscopy (SEM) and transmission electron microscopy (TEM), showing the spherical shape of AgNPs with particle sizes ranging from 18 to 39 nm. The silver presence in AgNPs was further confirmed by energy dispersive spectra. BiogenicAgNPs showed substantial antibacterial activity (24.21 ± 1.01 mm) for Xoo. In a pot experiment, AgNPs were found to be effective weapons for BLB by significantly increasing the plant biomass with a decreased cellular concentration of reactive oxygen species and increased concentration of antioxidant enzyme activity.
Entities:
Keywords:
B. cereus; BLB; antimicrobial activity; nanotechnology; rice; silver nanoparticles
Authors: Daiana S Guerrero; Romina P Bertani; Ana Ledesma; M de Los Angeles Frías; Cintia M Romero; José S Dávila Costa Journal: Heliyon Date: 2022-05-20
Authors: Natasha Manzoor; Liaqat Ali; Temoor Ahmed; Muhammad Noman; Muhammad Adrees; Muhammad Shafiq Shahid; Solabomi Olaitan Ogunyemi; Khlode S A Radwan; Gang Wang; Haitham E M Zaki Journal: Front Plant Sci Date: 2022-07-11 Impact factor: 6.627