| Literature DB >> 32100675 |
Léa Campos de Oliveira1, Natalia Bueno Pereira2, Carlos Henrique Valente Moreira3,2, Ana Luiza Bierrenbach4, Flavia Cristina Salles2, Marcela de Souza-Basqueira2, Erika Regina Manuli2, Ariela Mota Ferreira5, Cláudia Di Lorenzo Oliveira6, Clareci Silva Cardoso6, Antonio Luiz P Ribeiro7, Ester Cerdeira Sabino2.
Abstract
Chagas is a neglected disease endemic in Latin America. Vector transmission control had been aggressively performed. Recent entomological surveillance in Brazil has revealed natural infection rates ranging from 0.40% to 0.52%. Although serological surveys are complex to develop, they are important for disease control. In this study, we validated the use of saliva in ELISA commercial kits with a cohort of 100 patients with Chagas disease followed at Hospital das Clinicas in São Paulo, Brazil, and 50 healthy controls. Five ELISA kits for detecting antibodies against Trypanosoma cruzi were tested. The best discrimination between Chagas patients and controls was observed with the Wiener kit, which yielded a sensitivity of 97% and a specificity of 100%. Our findings reveal that the use of saliva may be an alternative to large-scale screening surveys in detecting T. cruzi antibodies; it is a noninvasive sample collection method potentially key to large-scale screening in children.Entities:
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Year: 2020 PMID： 32100675 PMCID： PMC7124906 DOI： 10.4269/ajtmh.18-0330
Source DB: PubMed Journal: Am J Trop Med Hyg ISSN： 0002-9637 Impact factor: 2.345