| Literature DB >> 26853491 |
Mini P Singh1, Manasi Majumdar2, Kapil Goyal3, P V M Lakshmi4, Deepak Bhatia5, R K Ratho6.
Abstract
Hepatitis E (HEV) infection is diagnosed on the basis of serum anti-HEV IgM detection. In outbreaks, early diagnostic method is important for prompt control measures. This study compared 3 diagnostic methods in 60 serum samples collected in suspected HEV outbreaks. The suitability of saliva samples for antibody detection was also evaluated in 21 paired serum saliva samples. The anti-HEV IgM, HEV-Ag, and HEV-RNA were detected in serum samples of 52 (86.66%), 16 (26.66%), and 18 (30%) patients, respectively. The concordance between serum and saliva IgM was found to be 76.91%. The positivity of PCR and HEV-Ag detection was 100% within 1 week of illness which declined to 5-10% thereafter. The outbreak was attributed to HEV genotype 1, subtype 1a, and the clinical and environmental strains clustered together. HEV-antigen and RNA were an early diagnostic marker with 96.66% concordance. Saliva samples can be used as an alternative in outbreak setting.Entities:
Keywords: HEV-antigen; Hepatitis E virus; Outbreak; Phylogenetic analysis; Saliva
Mesh：
Substances：
Year: 2015 PMID： 26853491 DOI： 10.1016/j.diagmicrobio.2015.12.002
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803