Mei Qu1, Maojun Zhang2, Xin Zhang3, Lei Jia4, Jun Xu5, Yanhui Chu5, Zhichao Liang4, Bing Lv4, Hao Liang2, Ying Huang4, Quanyi Wang6. 1. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, China. meiqu@126.com. 2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, 102206, China. 3. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, China. zhangxin@163.com. 4. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, China. 5. Beijing Xicheng District Center for Diseases Control and Prevention, China. 6. Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Institute of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing, 100013, China. bjcdcxm@126.com.
Abstract
INTRODUCTION: Campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China. In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China. The investigation for the enteric pathogen infection was conducted to identify the cause. METHODOLOGY: Eighteen stool specimens from 11 diarrheal patients and 3 close contacts were collected and tested for 16 enteric bacterial and viral pathogens using real-time PCR methods. Multilocus Sequence Typing (MLST), pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing were applied to C. jejuni isolates in this outbreak in order to characterize the incident. RESULTS: Ten (90.9%) of 11 stool specimens from diarrheal patients were positive for Campylobacter jejuni, confirmed as the cause of outbreak. Eight C. jejuni strains were obtained and produced 2 sequence types (STs) and 3 PFGE patterns. Six of them had the same ST (ST2274) and PFGE pattern (SMA001). The dominant outbreak strain with an identical subtyping profile was clustered with most chicken isolates from national MLST and PulseNet Campylobacter database. The entire 8 isolates were multi-drug resistant, with the dominant resistance pattern of nalidixic acid, tetracycline and ciprofloxacin combined, except for 2 isolates resistant to florfenicol. CONCLUSION: Molecular typing confirmed that most of the cases belonged to a clonal cluster supporting the hypothesis of a common source; however, the source was not identified. This was the first recognized Campylobacteriosis outbreak in China among 20 years. Copyright (c) 2019 Mei Qu, Maojun Zhang, Xin Zhang, Lei Jia, Jun Xu, Yanhui Chu, Zhichao Liang, Bing Lv, Hao Liang, Ying Huang, Quanyi Wang.
INTRODUCTION:Campylobacter spp. is the most common gastrointestinal pathogen worldwide with a very low reported incidence in China. In April 2018, one 36 cases of diarrhea outbreak occurred in a high school in Beijing after a trip to another province in Southern China. The investigation for the enteric pathogen infection was conducted to identify the cause. METHODOLOGY: Eighteen stool specimens from 11 diarrhealpatients and 3 close contacts were collected and tested for 16 enteric bacterial and viral pathogens using real-time PCR methods. Multilocus Sequence Typing (MLST), pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing were applied to C. jejuni isolates in this outbreak in order to characterize the incident. RESULTS: Ten (90.9%) of 11 stool specimens from diarrhealpatients were positive for Campylobacter jejuni, confirmed as the cause of outbreak. Eight C. jejuni strains were obtained and produced 2 sequence types (STs) and 3 PFGE patterns. Six of them had the same ST (ST2274) and PFGE pattern (SMA001). The dominant outbreak strain with an identical subtyping profile was clustered with most chicken isolates from national MLST and PulseNet Campylobacter database. The entire 8 isolates were multi-drug resistant, with the dominant resistance pattern of nalidixic acid, tetracycline and ciprofloxacin combined, except for 2 isolates resistant to florfenicol. CONCLUSION: Molecular typing confirmed that most of the cases belonged to a clonal cluster supporting the hypothesis of a common source; however, the source was not identified. This was the first recognized Campylobacteriosis outbreak in China among 20 years. Copyright (c) 2019 Mei Qu, Maojun Zhang, Xin Zhang, Lei Jia, Jun Xu, Yanhui Chu, Zhichao Liang, Bing Lv, Hao Liang, Ying Huang, Quanyi Wang.