Juliano Borges1,2,3, Luciana Araújo1,2,3, Tullia Cuzzi1,2,3, Luis Martinez1,2,3, Yliana Gonzales1,2,3, Mônica Manela-Azulay1,2,3. 1. Drs. Borges, Cuzzi, and Manela-Azulay are with Universidade Federal do Rio de Janeiro, UFRJ, in Rio de Janeiro-RJ, Brazil. 2. Dr. Araújo is with Universidade Federal do Estado do Rio de Janeiro, UNIRIO, Brazil. 3. Drs. Martinez and Gonzales are with Santa Casa de Misericórdia do Rio de Janeiro, Brazil.
Abstract
BACKGROUND: Laser resurfacing is a common treatment for photoaging. This treatment creates skin incisions that initiate the wound healing cascade, including reorganization of the collagen matrix, in a dermal remodeling process that can last up to 12 months. OBJECTIVE: We compared the effects of nonablative and ablative Erbium fractional laser resurfacing on dermal content and arrangement of Collagen Types I and III, and on fibroblast activation. METHODS: Ten female patients (50-63 years) with Fitzpatrick Skin Types I-IV and clinical signs of photoaging underwent two types of Erbium fractional laser resurfacing (nonablative, 1540nm; ablative, 2940nm) on opposite sides of the face. Skin biopsies were obtained pretreatment and three months post-treatment. Morphometric analysis was performed using Picrosirius staining for overall collagen, and immunohistochemistry for Collagen Types I and III. Finally, hematoxylin and eosin staining was used to identify fibroblast activation. RESULTS: Both laser treatments induced reorganization of Collagen Types I and III and demonstrated signs of fibroblast activation. However, morphometric analysis of Picrosirius staining revealed that, after both treatments, there was a lower density of collagen fibers, which is characteristic of edema. CONCLUSION: At three months after laser resurfacing, skin lifting in photoaged skin likely resulted from new collagen deposition but also from edema.
BACKGROUND: Laser resurfacing is a common treatment for photoaging. This treatment creates skin incisions that initiate the wound healing cascade, including reorganization of the collagen matrix, in a dermal remodeling process that can last up to 12 months. OBJECTIVE: We compared the effects of nonablative and ablative Erbium fractional laser resurfacing on dermal content and arrangement of Collagen Types I and III, and on fibroblast activation. METHODS: Ten female patients (50-63 years) with Fitzpatrick Skin Types I-IV and clinical signs of photoaging underwent two types of Erbium fractional laser resurfacing (nonablative, 1540nm; ablative, 2940nm) on opposite sides of the face. Skin biopsies were obtained pretreatment and three months post-treatment. Morphometric analysis was performed using Picrosirius staining for overall collagen, and immunohistochemistry for Collagen Types I and III. Finally, hematoxylin and eosin staining was used to identify fibroblast activation. RESULTS: Both laser treatments induced reorganization of Collagen Types I and III and demonstrated signs of fibroblast activation. However, morphometric analysis of Picrosirius staining revealed that, after both treatments, there was a lower density of collagen fibers, which is characteristic of edema. CONCLUSION: At three months after laser resurfacing, skin lifting in photoaged skin likely resulted from new collagen deposition but also from edema.
Authors: J Varani; D Spearman; P Perone; S E Fligiel; S C Datta; Z Q Wang; Y Shao; S Kang; G J Fisher; J J Voorhees Journal: Am J Pathol Date: 2001-03 Impact factor: 4.307
Authors: J H Chung; J Y Seo; H R Choi; M K Lee; C S Youn; G Rhie; K H Cho; K H Kim; K C Park; H C Eun Journal: J Invest Dermatol Date: 2001-11 Impact factor: 8.551
Authors: Lene Hedelund; Christina S Haak; Katrine Togsverd-Bo; Morten K Bogh; Peter Bjerring; Merete Haedersdal Journal: Lasers Surg Med Date: 2012-07-05 Impact factor: 4.025