| Literature DB >> 32079746 |
Jong-Eun Park1, Rachel A Botting2, Cecilia Domínguez Conde1, Dorin-Mirel Popescu2, Marieke Lavaert3,4, Daniel J Kunz1,5,6, Issac Goh2, Emily Stephenson2, Roberta Ragazzini7,8, Elizabeth Tuck1, Anna Wilbrey-Clark1, Kenny Roberts1, Veronika R Kedlian1, John R Ferdinand9, Xiaoling He10, Simone Webb2, Daniel Maunder2, Niels Vandamme11,12, Krishnaa T Mahbubani13, Krzysztof Polanski1, Lira Mamanova1, Liam Bolt1, David Crossland2,14, Fabrizio de Rita14, Andrew Fuller2, Andrew Filby2, Gary Reynolds2, David Dixon2, Kourosh Saeb-Parsy13, Steven Lisgo2, Deborah Henderson2, Roser Vento-Tormo1, Omer A Bayraktar1, Roger A Barker10,15, Kerstin B Meyer1, Yvan Saeys11,12, Paola Bonfanti7,8,16, Sam Behjati1,17, Menna R Clatworthy1,9,18, Tom Taghon19,4, Muzlifah Haniffa20,2,21, Sarah A Teichmann20,5.
Abstract
The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα+ T cell populations, thymic fibroblast subtypes, and activated dendritic cell states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and the kinetics of lineage commitment. Taken together, our data provide a comprehensive atlas of the human thymus across the life span with new insights into human T cell development.Entities:
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Year: 2020 PMID： 32079746 PMCID： PMC7611066 DOI： 10.1126/science.aay3224
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728